Protein Info for Sama_3313 in Shewanella amazonensis SB2B

Annotation: cold-active alkaline serine protease (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 514 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF22148: Fervidolysin_NPro-like" amino acids 29 to 84 (56 residues), 22.4 bits, see alignment 2e-08 PF00082: Peptidase_S8" amino acids 113 to 319 (207 residues), 108 bits, see alignment E=8.6e-35 amino acids 417 to 478 (62 residues), 59.6 bits, see alignment E=4.9e-20 PF02225: PA" amino acids 337 to 405 (69 residues), 48.6 bits, see alignment E=1.1e-16

Best Hits

KEGG orthology group: None (inferred from 100% identity to saz:Sama_3313)

Predicted SEED Role

"Cold-active alkaline serine protease (EC 3.4.21.62)" (EC 3.4.21.62)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.62

Use Curated BLAST to search for 3.4.21.62

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1SAV9 at UniProt or InterPro

Protein Sequence (514 amino acids)

>Sama_3313 cold-active alkaline serine protease (RefSeq) (Shewanella amazonensis SB2B)
MQSIYKPLAAAVTLALCSGAYAAEQERYIVKFKEGKGPSVMAQLKSQGGKMALDLSSHNA
AAFTLPAKALKGLENNPNVEYVEVDAKRYPLGQVVPYGIPMVQADLLSDAATSNTTVCII
DSGYELAHEDLAGNQVTGTNDAGTGNWFTDENHHGTHVAGTIAALNNNTGVVGVNPNGNL
NLHIIKVFNADGWGYSSSLVSALNKCEQAGAKVINMSLGGSRSSRTEDSAFAAAESRGVL
SIAAAGNDGNTRHSYPASYNSVVSVAAIDSNKVVADFSQKTSQVELSGPGVGVLSSVPTG
TALVASTTVAGTGYEAIGMEGSPTGDASGMLADCGTGETACNANGQVCLIQRGVISFAEK
VQACENGGGVAAIIYNNVSGALNGTMGGVATSIPSVGVSDVDGAAMLAQVGSNASVSIGA
GNYAYFDGTSMATPHVAGVAALVWSHFPQCSNTQIRAALRATAEDLGVAGRDDSYGYGLV
KAKDAVDYLTTYGCDGQSSGGGNTGGTCKGRNCK