Protein Info for Sama_3296 in Shewanella amazonensis SB2B

Annotation: aconitate hydratase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02333: 2-methylisocitrate dehydratase, Fe/S-dependent
  • amino acids 2 to 859 (858 residues), 1769.8 bits, E=0
• PF00330: Aconitase
  • amino acids 69 to 533 (465 residues), 462.1 bits, E=2.5e-142
• PF00694: Aconitase_C
  • amino acids 656 to 787 (132 residues), 98.2 bits, E=5.1e-32

Best Hits

Swiss-Prot: 82% identical to ACND_SHEON: 2-methylcitrate dehydratase (2-methyl-trans-aconitate forming) (acnD) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01681, aconitate hydratase 1 [EC: 4.2.1.3] (inferred from 84% identity to amc:MADE_03372)

MetaCyc: 82% identical to 2-methylcitrate dehydratase (Shewanella oneidensis)
RXN-8979 [EC: 4.2.1.117]

Predicted SEED Role

"2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 4.2.1.79)

MetaCyc Pathways

• mixed acid fermentation (15/16 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• TCA cycle III (animals) (9/10 steps found)
• glyoxylate cycle (6/6 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• 2-methylcitrate cycle I (5/5 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• nitrogen remobilization from senescing leaves (7/8 steps found)
• ethene biosynthesis V (engineered) (19/25 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• 2-methylcitrate cycle II (5/6 steps found)
• TCA cycle V (2-oxoglutarate synthase) (7/9 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• partial TCA cycle (obligate autotrophs) (6/8 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (6/9 steps found)
• reductive TCA cycle I (7/11 steps found)
• TCA cycle VII (acetate-producers) (5/9 steps found)
• reductive TCA cycle II (6/12 steps found)
• methylaspartate cycle (9/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Propanoate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.3

Use Curated BLAST to search for 4.2.1.117 or 4.2.1.3 or 4.2.1.79

Sequence Analysis Tools

See A1SAU2 at UniProt or InterPro

Protein Sequence (861 amino acids)

>Sama_3296 aconitate hydratase (RefSeq) (Shewanella amazonensis SB2B)
MNTQFRKPLPGTNLSFFDTREAVDAIAPGAFDKLPYTSKVLAENLLRKAEPARLNDFLSQ
LIFRKQDLDFPWFPARVVCHDILGQTALVDLAGLRDAIAAKGGDPAKVNPVVPTQLIVDH
SLAVEHGGFEKDAFEKNRAIEDRRNDDRFHFINWTKKAFRNVDVIPPGNGIMHQINLEKM
SPVIQVRDGIAFPDTCVGTDSHTPHVDSLGVIAIGVGGLEAENVMLGRASWMRLPDIVGV
ELTGKPNPGITATDVVLALTEFLRKERVVGAYLEFFGEGAKALTLGDRATISNMTPEYGA
TAAMFYIDEQTIDYLRLTGRDEKQVELVENYAKTTGLWADAMTGADYGRILTFDLSSVVR
NMAGPSNPHARLATSDLAAKGIAADWQEESGKMPDGAVIIAAITSCTNTSNPRNVIAAGL
IARNAVQKGLVRKPWVKTSLAPGSKAVELYLKEAGLLPYLEQLGFGIVAFACTTCNGMSG
ALDPVIQQEIIERDLYATAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAGTVRFDIE
KDVLGIDDAGNAVTLKDLWPEDAEIDAIIKSSVKPEQFRAVYDPMFNLAVEYGTEKPLYD
WRPQSTYIRRPPYWEGALAGERTLSGMRPLAVLGDNITTDHLSPSNAILASSAAGEYLAK
MGLPEEDFNSYATHRGDHLTAQRATFANPKLFNEMVKDADGKVKQGSLARLEPEGKVLRM
WETIETYMERKQPLIIVAGKDYGQGSSRDWAAKGVRLAGVEVIVAEGFERIHRTNLVGMG
VLPLEFMPGETRMTYGIDGTETFDVKGERTPGAELTLLIRRQNGETVEVPVRCRLDTAEE
VSIYEAGGVLQRFAQDFLANS