Protein Info for Sama_3246 in Shewanella amazonensis SB2B
Annotation: HAD-superfamily hydrolase, subfamily IA, variant 1 family protein (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 38% identical to YIGB_SHIFL: Flavin mononucleotide phosphatase YigB (yigB) from Shigella flexneri
KEGG orthology group: K07025, putative hydrolase of the HAD superfamily (inferred from 100% identity to saz:Sama_3246)MetaCyc: 38% identical to 5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase (Escherichia coli K-12 substr. MG1655)
RIBOPHOSPHAT-RXN [EC: 3.1.3.104]; RXN0-5187 [EC: 3.1.3.104, 3.1.3.102]
Predicted SEED Role
"Putative FMN hydrolase (EC 3.1.3.-); 5-Amino-6-(5'-phosphoribitylamino)uracil phosphatase" (EC 3.1.3.-)
MetaCyc Pathways
- flavin biosynthesis I (bacteria and plants) (9/9 steps found)
- flavin biosynthesis III (fungi) (8/9 steps found)
- toxoflavin biosynthesis (4/7 steps found)
- flavin biosynthesis II (archaea) (6/10 steps found)
KEGG Metabolic Maps
- Aminosugars metabolism
- Ascorbate and aldarate metabolism
- Ether lipid metabolism
- Fructose and mannose metabolism
- Lipopolysaccharide biosynthesis
- Nicotinate and nicotinamide metabolism
- Riboflavin metabolism
- Sphingolipid metabolism
- Thiamine metabolism
Isozymes
Compare fitness of predicted isozymes for: 3.1.3.-
Use Curated BLAST to search for 3.1.3.- or 3.1.3.102 or 3.1.3.104
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A1SAP2 at UniProt or InterPro
Protein Sequence (238 amino acids)
>Sama_3246 HAD-superfamily hydrolase, subfamily IA, variant 1 family protein (RefSeq) (Shewanella amazonensis SB2B) MQIFRRLKPIAAISFDLDDTLYDNRPVIMAAELAQLMYLARHYPVTKAWGRDEWRHHKLR VLAHHPVLAHDPTALRLLTLHSGLLYFGLSEADATTAAGAVMDEFIRARSHFRVPESSLQ LLAKLGQKYPLIGLTNGNVDPERIGLGDRLVFVLAAGSGVRMKPVDDLFRMGCNRLGIAS EQLLHVGDSHSADVVGALKAGCQAVLLNPAHGGVEPGPLASPLPHLQIQRLDALLALL