Protein Info for Sama_3153 in Shewanella amazonensis SB2B

Annotation: putative diguanylate phosphodiesterase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 584 PF00563: EAL" amino acids 10 to 240 (231 residues), 170.1 bits, see alignment E=8.5e-54 PF00571: CBS" amino acids 277 to 322 (46 residues), 16.6 bits, see alignment 1.2e-06 PF00990: GGDEF" amino acids 406 to 495 (90 residues), 50.5 bits, see alignment E=3.1e-17 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 416 to 494 (79 residues), 48.3 bits, see alignment E=4.5e-17

Best Hits

KEGG orthology group: None (inferred from 100% identity to saz:Sama_3153)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1SAE9 at UniProt or InterPro

Protein Sequence (584 amino acids)

>Sama_3153 putative diguanylate phosphodiesterase (RefSeq) (Shewanella amazonensis SB2B)
MMAAIDLKAELTKLIEKQSIESLFQPIFNISAGKIHGFEALSRGPVHSPLYSPVPLFKTA
EHEGRLSELETLCRQIAAGRFAERKLPGKLFINISPKALLDPDHPKGKTLTMLQQLGISP
SQVVIELSEQHPADDIDLLKTCLLHYRNQGFLTAIDDLGAGYSGLRLWSELAPDYVKIDR
HFIHQIDKHPVKQEFVRSIVELCQSLTCKVIAEGIETAAELQVLRQLGIIYCQGFLLGRP
EANPNKQLPPGLSPQPNIGQSTHRYIETAESLACGAVTVPTDTKLKTISDRFSSQPALQA
MVVVEGDTPLGLISRTALLELFSTPYGRALHENHCVTEVMDTRVPQFDASEALSSVSQLL
TSDSDQLMAQQFLILRQDKLIGVGHTKDLLARITEHRIKVARHANPLTGLPGNVPIQEEL
QRLKQQSRPFYLVYFDLSHFKPYNDIYGFCRGDEVICYVASLLAEHQDADCFVGHIGGDD
FVMITTDDPTCRVAGILKNFEAEKRSFFQEAHWQAQFISAEDRQGALSLQPLTSLCAGIL
SPRHTFNATEHELSALSARAKKQAKLTESGLCLLGSTPPQLRQA