Protein Info for Sama_3146 in Shewanella amazonensis SB2B
Annotation: formate--tetrahydrofolate ligase (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to FTHS_SHEAM: Formate--tetrahydrofolate ligase (fhs) from Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)
KEGG orthology group: K01938, formate--tetrahydrofolate ligase [EC: 6.3.4.3] (inferred from 100% identity to saz:Sama_3146)Predicted SEED Role
"Formate--tetrahydrofolate ligase (EC 6.3.4.3)" in subsystem Folate Biosynthesis or One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle (EC 6.3.4.3)
MetaCyc Pathways
- folate transformations II (plants) (11/11 steps found)
- folate transformations III (E. coli) (9/9 steps found)
- folate polyglutamylation (5/5 steps found)
- superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
- formaldehyde oxidation VII (THF pathway) (4/4 steps found)
- formate assimilation into 5,10-methylenetetrahydrofolate (3/3 steps found)
- folate transformations I (10/13 steps found)
- tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
- L-histidine degradation III (4/6 steps found)
- reductive glycine pathway of autotrophic CO2 fixation (6/9 steps found)
- reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (4/10 steps found)
- purine nucleobases degradation I (anaerobic) (5/15 steps found)
- purine nucleobases degradation II (anaerobic) (11/24 steps found)
- Methanobacterium thermoautotrophicum biosynthetic metabolism (22/56 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.3.4.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A1SAE2 at UniProt or InterPro
Protein Sequence (560 amino acids)
>Sama_3146 formate--tetrahydrofolate ligase (RefSeq) (Shewanella amazonensis SB2B) MLTDMEISRQANVRPIGWIADDFGLGKHLWREAGHAMAKISLEAARAPLRGKLVIVTAVT PTPHGEGKTVTTIGLTQGLHLLGHKACACIRQPSLGPVFGVKGGAAGGGYAQVVPMEVMN LHMTGDIHAVTSAHNLAAAAIDARLFHETRLGCESFSAKTGLPALNIDPERILWRRVVDH NDRSLRQIELGLGDNNGPVHGGGFDITAASELMAILALCENIADMRARIGRVILAMSRDG KPISADDLGVAGAMTAIMRDAIEPTLMQTLSGAPCLVHAGPFANIAHGNSSIIADRVALA HHDYVVTEGGFGSDMGFEKFCNIKSRASGIAPSAAVLVVTLRALKANSGVEGDDRARLEA GFANLHWHMQNVMGYGLPLVVAINRFPDDAMAELDWLKERCLEEGAFGCEISDAFSHGAE GARALATAVARACDAPVNFEYLYDDEMDFGARLMTLAERYGAAGVSLSEDARRDLAMITE LGMDKLPLCMAKTPLSISHDPSLKGAPSGFELPVVGLRLSAGAGFITVLTGKVMTMPGLG LLPGYLNIDINDKGEIIGLG