Protein Info for Sama_3055 in Shewanella amazonensis SB2B

Annotation: tRNA-dihydrouridine synthase A (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00742: tRNA dihydrouridine synthase A
  • amino acids 8 to 322 (315 residues), 513.4 bits, E=1.3e-158
• PF01207: Dus
  • amino acids 11 to 313 (303 residues), 305.3 bits, E=2.2e-95

Best Hits

Swiss-Prot: 80% identical to DUSA_SHEON: tRNA-dihydrouridine(20/20a) synthase (dusA) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K05539, tRNA-dihydrouridine synthase A [EC: 1.-.-.-] (inferred from 100% identity to saz:Sama_3055)

MetaCyc: 71% identical to tRNA-dihydrouridine synthase A (Escherichia coli K-12 substr. MG1655)
1.1.1.-

Predicted SEED Role

"tRNA dihydrouridine synthase A"

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Carotenoid biosynthesis - General
• Insect hormone biosynthesis
• Nucleotide sugars metabolism
• Porphyrin and chlorophyll metabolism
• Puromycin biosynthesis
• Trinitrotoluene degradation
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

See A1SA51 at UniProt or InterPro

Protein Sequence (326 amino acids)

>Sama_3055 tRNA-dihydrouridine synthase A (RefSeq) (Shewanella amazonensis SB2B)
MPSPLNRTFSVAPMLDWTDRHYRYFARLMSKEALLYTEMVTTGAILHGKGDYLAYNQEEH
PLALQLGGSDPKDLATCAKLAEARGYDEVNINVGCPSDRVQNGRFGACLMAEPKLVAECV
DAMRQQVSVPVTVKTRIGIDEQDSYGFLTDFVETVSAAGCDTFIIHARKAWLQGLSPKEN
REIPPLDYERVYQLKRDYPQLSISINGGVTSIEQAKTHLAQLDGVMVGREAYQNPYILAS
VDTELCGKPAVVSSREEVIGKLLPYIGKHLADGGRLNHISRHIIGLYQGLPGSRAWRRHL
SENAHKPGAGIEVIENAIKAMELTTN