Protein Info for Sama_3048 in Shewanella amazonensis SB2B

Name: tolC
Annotation: outer membrane channel protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 432 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details TIGR01844: type I secretion outer membrane protein, TolC family" amino acids 22 to 427 (406 residues), 351.4 bits, see alignment E=3.6e-109 PF02321: OEP" amino acids 27 to 207 (181 residues), 105.3 bits, see alignment E=1.8e-34 amino acids 232 to 414 (183 residues), 127.6 bits, see alignment E=2.7e-41

Best Hits

Swiss-Prot: 50% identical to TOLC_VIBCH: Outer membrane protein TolC (tolC) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K12340, outer membrane channel protein TolC (inferred from 100% identity to saz:Sama_3048)

MetaCyc: 50% identical to outer membrane channel TolC (Vibrio cholerae O1 biovar El Tor str. N16961)
TRANS-RXN-502

Predicted SEED Role

"Type I secretion outer membrane protein, TolC precursor" in subsystem Multidrug Resistance Efflux Pumps or Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1SA44 at UniProt or InterPro

Protein Sequence (432 amino acids)

>Sama_3048 outer membrane channel protein (RefSeq) (Shewanella amazonensis SB2B)
MKFKFSSLCAAIALAASASSVQADDLLQIYQQALTNDPLVLQAQAQRNALYAQIEENRAP
LLPTISANVGYDKAWKDPSEDSDGFTGGVKLTQVIYDHSAWVGLSLAEKAAAQADAAYAS
ALQNLITRVTKAYFDVLKAKDNYEFQGAEKRAIERQLEQTKQRFAVGLTAMTDVHEAQAQ
YDLASATEILAENTLSNSYEALREITGIDHKSINVLDTNRFSAAAPAPAAPNEWIKMAEN
NSTDLLTQRIGKDIAEETISLYKAGHYPSLSLSAGYTKGFEQTPGPDYDNSSIGVTLSIP
IFEGFKVTSKVEQAQYKYVEASEKLEQTYRRVVKDVRNNFNNVGASISSIRAYEQSVLSS
ESALGATQSGFEVGTRTIVDVLNRTRDLYDSKRKLSDARYSYISSVLALKQAAGTLNEDD
VIAINNGLKAAE