Protein Info for Sama_3024 in Shewanella amazonensis SB2B

Annotation: GTP-binding protein HflX (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03156: GTP-binding protein HflX
  • amino acids 11 to 359 (349 residues), 511 bits, E=7e-158
• PF13167: GTP-bdg_N
  • amino acids 25 to 112 (88 residues), 109.2 bits, E=3.2e-35
• PF16360: GTP-bdg_M
  • amino acids 114 to 192 (79 residues), 106 bits, E=2.9e-34
• PF00025: Arf
  • amino acids 199 to 325 (127 residues), 27.2 bits, E=6.3e-10
• PF01926: MMR_HSR1
  • amino acids 200 to 317 (118 residues), 81.5 bits, E=1.3e-26

Best Hits

Swiss-Prot: 66% identical to HFLX_ECOLI: GTPase HflX (hflX) from Escherichia coli (strain K12)

KEGG orthology group: K03665, GTP-binding protein HflX (inferred from 100% identity to saz:Sama_3024)

MetaCyc: 66% identical to ribosome rescue factor HflX (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein HflX" in subsystem Hfl operon or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See A1SA20 at UniProt or InterPro

Protein Sequence (435 amino acids)

>Sama_3024 GTP-binding protein HflX (RefSeq) (Shewanella amazonensis SB2B)
MFDRYEAGETAVLVHIDFTDEGSREDLAELQLLVESAGARSVGVITGSRRAPDRKFFVGT
GKAEELAAMVAATDANVVIFNHALSPAQERNLEHLCRCRVLDRTTLILDIFAQRARTHEG
KLQVELAQLRHMSTRLVRGWTHLERQKGGIGLRGPGETQLETDRRLLRGRIKNINRRLEK
VDKQREQSRRARQRSDMAAVSLVGYTNAGKSTLFNTLTSAEVYAADQLFATLDPTLRKLE
LPDGAAILADTVGFIRHLPHDLVAAFKATLQETREADLLLHVIDCADEKMRDNIEQVQAV
LKEIDADEIPQLIVCNKIDLVEGIKPAIDRDDTGKPFRVWLSAQQNQGLDLLRQAVQELV
GNVIAELTLKIPATAGHYLGQFYRLDAIQQKEYDDLGNCILSVRLPDADWRRLAKQSQGE
LETFIVDTATDRVVC