Protein Info for Sama_3010 in Shewanella amazonensis SB2B

Updated annotation (from data): Arginine N-succinyltransferase (EC 2.3.1.109)
Rationale: Specifically important for utilizing L-Arginine. Automated validation from mutant phenotype: the predicted function (ARGININE-N-SUCCINYLTRANSFERASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: arginine N-succinyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04958: AstA
  • amino acids 1 to 337 (337 residues), 465 bits, E=5.1e-144
• TIGR03244: arginine N-succinyltransferase
  • amino acids 3 to 338 (336 residues), 464.2 bits, E=2e-143
• TIGR03243: arginine and ornithine succinyltransferase subunits
  • amino acids 3 to 338 (336 residues), 447.4 bits, E=2.7e-138

Best Hits

Swiss-Prot: 47% identical to ASTA_YERE8: Arginine N-succinyltransferase (astA) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: K00673, arginine N-succinyltransferase [EC: 2.3.1.109] (inferred from 100% identity to saz:Sama_3010)

Predicted SEED Role

"Arginine N-succinyltransferase (EC 2.3.1.109)" in subsystem Arginine and Ornithine Degradation (EC 2.3.1.109)

MetaCyc Pathways

• L-arginine degradation II (AST pathway) (5/5 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.109

Use Curated BLAST to search for 2.3.1.109

Sequence Analysis Tools

See A1SA06 at UniProt or InterPro

Protein Sequence (339 amino acids)

>Sama_3010 Arginine N-succinyltransferase (EC 2.3.1.109) (Shewanella amazonensis SB2B)
MLIIRPIRASDFDALYRIAVESGHGFTSLPVNEDLLRRKIARSEASFQKVVETPYDEGYL
MVLEDTQTQEVVGTCALEAAVGMEDAFYHYRLGTEVYHSQQINVRNEVETLTLCHDYTGA
AELCTLFLRGAYRKDNNGRMLSRSRFLFLAQHANRFGETVIAEMRGVSDENGNSPFYGWL
QKHFLGIDFVEADYLSGLGRKAFMAEMMPRNPVYVCLLPEEAQKVIGEVHTNTRPALSLL
RAEGFRCRGYVDIFDGGPTVECNLQDIRGVRESRLLTVRIGEMPASDDSFILSNTQLVDY
RATSVELMVSSDSDEVILSPELAAGLLVSDGEQVRVLAI