Protein Info for Sama_2971 in Shewanella amazonensis SB2B

Annotation: peptidoglycan glycosyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 779 transmembrane" amino acids 38 to 58 (21 residues), see Phobius details TIGR02071: penicillin-binding protein 1B" amino acids 40 to 758 (719 residues), 899.5 bits, see alignment E=7.6e-275 PF14814: UB2H" amino acids 85 to 169 (85 residues), 82.1 bits, see alignment E=3.6e-27 PF00912: Transgly" amino acids 181 to 350 (170 residues), 198.1 bits, see alignment E=1.2e-62 PF00905: Transpeptidase" amino acids 444 to 692 (249 residues), 83.1 bits, see alignment E=3.3e-27

Best Hits

Swiss-Prot: 49% identical to PBPB_VIBCH: Penicillin-binding protein 1B (mrcB) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K05365, penicillin-binding protein 1B [EC: 2.4.1.129 3.4.-.-] (inferred from 100% identity to saz:Sama_2971)

MetaCyc: 44% identical to peptidoglycan glycosyltransferase / peptidoglycan DD-transpeptidase MrcB (Escherichia coli K-12 substr. MG1655)
Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.4.16.4]; 3.4.16.4 [EC: 3.4.16.4]; Peptidoglycan glycosyltransferase. [EC: 3.4.16.4, 2.4.1.129]

Predicted SEED Role

"Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)" in subsystem Peptidoglycan Biosynthesis or Type IV pilus (EC 2.4.1.129, EC 3.4.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129, 3.4.-.-, 3.4.16.4

Use Curated BLAST to search for 2.4.1.129 or 3.4.-.- or 3.4.16.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S9W7 at UniProt or InterPro

Protein Sequence (779 amino acids)

>Sama_2971 peptidoglycan glycosyltransferase (RefSeq) (Shewanella amazonensis SB2B)
MTSDKRNSGKRAPAKGASAKSRTSAKARNKPGILRRIWSFLWKLTLVVLVLCAAYGIYLD
QQIARKFEGQKWHLPAQIYSRSMALYPGAAVSHHQLLAELQLLGYRKVANPRQVGEFSAS
ATRIDLWRRPFLHPLGSQDAARVMIAFDSQGVSTVSRHGDKRELAVFHLEPMLLDRLIAD
DGEDRLFVPRREVPDTMVEALLLVEDRSFYEHHGVNPFAILRAAFVNISAGRTVQGGSTL
TQQLAKNFFLSSERSLIRKVREALMAIIIDFRYSKDEILEAYLNEVYMGQDKSRGVHGMG
LASQFYFGRPLTELTTAQQAFLIAVIKGPSYYNPWRYPARAQERRDLVLRLLMESGKISV
NDFQIAAESPLGLRPEGRPVHQRMPAFYTQVKKELALRWGDSLLNESGIKVYTTLDPIAQ
EAAEKAVKEVMGRIGKDTKELQAAMVVTDKFGGGIAAMVGDRNPSFEGYNRVSEMRRPIG
SLVKPFVYMTALEDSNYNLMTSLKDEPLTVTNKHGQSWSPMNYDKTFSGQVPLMRAMYKS
LNVPTVNLGLAVGVDAVINTLDDAGWRGEAINPYPSMLLGAVDGSPLMVAQIYQTLADNG
RYRQLSTVTDVLDMNNEAILASRLPSEQSLEPAAAYLSQYIMTRVVSEGTGARLGRAFPN
VTLAGKTGTSNDSRDSWFAGFDERNVAAVWVGRDDNGKTKLVGASGAMAIYQAFLEARPP
VALRMTPPDDVVQGYFDLNGNALEKGCPGAVALPAKAGSWRPAQNCGKPLSWWQKIIGG