Protein Info for Sama_2795 in Shewanella amazonensis SB2B

Annotation: transketolase, central region (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 761 PF00676: E1_dh" amino acids 67 to 358 (292 residues), 102.3 bits, see alignment E=3.8e-33 PF02779: Transket_pyr" amino acids 402 to 577 (176 residues), 79.3 bits, see alignment E=4.3e-26 PF02780: Transketolase_C" amino acids 617 to 712 (96 residues), 62.6 bits, see alignment E=5.3e-21

Best Hits

KEGG orthology group: K11381, 2-oxoisovalerate dehydrogenase E1 component [EC: 1.2.4.4] (inferred from 100% identity to saz:Sama_2795)

Predicted SEED Role

"Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4) / Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4)" in subsystem Isoleucine degradation or Leucine Degradation and HMG-CoA Metabolism or Valine degradation (EC 1.2.4.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.4.4

Use Curated BLAST to search for 1.2.4.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S9E1 at UniProt or InterPro

Protein Sequence (761 amino acids)

>Sama_2795 transketolase, central region (RefSeq) (Shewanella amazonensis SB2B)
MEDRAIAVERQFIEALETGSVGAFIDNTQGWDHRRLGLSDAEFVGIFESQLKSRLLDLES
RRMRARNEGFYTIGSSGHEGNAAIAAVLNTTDMALLHYRSGAFMVERSRHEASETVLWDM
MLSFAASSEDPISGGRHKVLGSKSLNIPPQTSTIASHLPKAVGVALSIPLTERLGVDGVL
PPDAIAMCNFGDASANHASAQTAINAACWAAFQNIPMPLMFVCEDNGIGISTPTPKGWIG
ANFSQRPGLKYFCCDGLDLLDTYKVAKEAAHWCRSHRQPVFLHVRTVRLMGHAGSDAEIA
YLPKAKILENEALDPLLRSAAMLIEAGVLSAEQILAMYQELKDRIAAIARVAATRPKLTT
AKDAMASVVPPKLANPRAVKTLDEEAFASLFAADKQSLGKPVHMGKLINLTLTELMASHD
NVVVCGEDVGKKGGVYHVTSRLVERFGPSRVINTLLDETSILGLATGMAHNGLLPIPEIQ
FLAYVHNAEDQIRGEAATLPFFSNGQYTNPMVIRIAGLAYQKGFGGHFHNDNSFTVFRDI
PGLILACPSNGADAQGMLRECVRLAREEQRLVIFLEPIALYMTRDLHEPGDSLWAAQYVP
EREATPLPFGEPGRFGEGKDLCIISYGNGYYLSRQAEKALAEAGIDCTLVDLRYLAPLNE
AAICDIAANCRHVLVVDECRRSGSVSEAIVTALHERLGSACPKLARLNAEDCFIPLADAA
TLPLPGKDSIVAAALKLVQGSDAGTGKGTDTGIVIGLEASA