Protein Info for Sama_2663 in Shewanella amazonensis SB2B

Annotation: (glutamate--ammonia-ligase) adenylyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 961 PF03710: GlnE" amino acids 42 to 276 (235 residues), 286.5 bits, see alignment E=1.8e-89 amino acids 558 to 809 (252 residues), 304.8 bits, see alignment E=4.6e-95 PF08335: GlnD_UR_UTase" amino acids 307 to 444 (138 residues), 87.3 bits, see alignment E=1e-28 amino acids 831 to 922 (92 residues), 31 bits, see alignment E=2.3e-11

Best Hits

Swiss-Prot: 100% identical to GLNE_SHEAM: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 100% identity to saz:Sama_2663)

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S909 at UniProt or InterPro

Protein Sequence (961 amino acids)

>Sama_2663 (glutamate--ammonia-ligase) adenylyltransferase (RefSeq) (Shewanella amazonensis SB2B)
MSQQHQAEIPKEIKEKGSLWLASLEAHHPESLTGLDTVQRRELEQVAGLSDFIARELSRH
PERIARLFDGLLDKVDRSAFDRELHQALASVTSEDEAKAVLRRYRNFQMARLAWRDFTGK
APLGESLLDISSLAEALIITGRDWLYREMCQSLGTPMDADGRPQPLMILGMGKLGGRELN
FSSDIDLIFTFPEHGETQGGRKSLDNQQFFIRMGQRLVNLLSQVTVDGFVFRVDMRLRPY
GESGPLVVSFSALEDYYQEQGRDWERYAMVKARALGPWSGYSDELHSLLRPFVYRRYIDF
SAIESLRRMKQLIAQEVRRRHLTDNIKLGAGGIREVEFVVQSFQLIRGGREPSLRTQSLF
GAIDTLYSLGQLEYLAVDELKNAYVLLRRAENLLQAIDDKQTQTLPDNSLDWQRLCCALE
MANEFDLRAHIEAAMAKIHRHFRATVGGEEAEDNHEHWTWQFWNVQEEDHAEALLQDQHI
DDPALWPLLKGWRETMSRRPIGPRGRETLDRLMPKLLGELVSMPIPSAAFAPVFNVVEQV
LTRTTYLELLCENPGARAQLVSLCCASPWIADQLAKFPMLLDELIDPAALYDITSLDDYG
SELRQYLLRVPEDDMEQQMEALRQFKLTQQLKIAAADVTGVLPIMQVSDHLTFLAEAIIE
QVVHQAWGQVASRHGVPAHLESGQMGFAVIAYGKLGGIELGYGSDLDLVFLHNYQGSGLT
TGERQIETSHFYLKLAQRILHLFSTRTTSGELYEVDMRLRPSGASGLLVSEIERFGEYQQ
QEAWTWEHQALVRSRFVFGDNQLAARFSEIRAEVLGKVRDPAVLKGEVKAMRTKMREHLL
KVDEGMFDLKQSPGGIADIEFIAQYLVLAGSAEFEDLTVWSDNVRIFQTAAELDILPLPV
AQQLIAAYCTLRDENHRLTLQREAGQLPAIAVAEHSERVCAIYADVLGHDTDEPPLESRS
Q