Protein Info for Sama_2636 in Shewanella amazonensis SB2B
Updated annotation (from data): gamma-aminobutyrate transaminase (EC 2.6.1.19)
Rationale: Specifically improtant for putrescine utilization
Original annotation: 4-aminobutyrate aminotransferase (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 70% identical to DAVT_PSEAE: 5-aminovalerate aminotransferase DavT (davT) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K07250, 4-aminobutyrate aminotransferase / (S)-3-amino-2-methylpropionate transaminase [EC: 2.6.1.19 2.6.1.22] (inferred from 100% identity to saz:Sama_2636)MetaCyc: 70% identical to 5-aminovalerate aminotransferase (Pseudomonas aeruginosa)
5-aminovalerate transaminase. [EC: 2.6.1.48]
Predicted SEED Role
"Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19)" (EC 2.6.1.19)
MetaCyc Pathways
- 4-aminobutanoate degradation III (2/2 steps found)
- L-valine degradation I (6/8 steps found)
- GABA shunt II (3/4 steps found)
- 4-aminobutanoate degradation I (1/2 steps found)
- 4-aminobutanoate degradation II (1/2 steps found)
- GABA shunt I (2/4 steps found)
- superpathway of 4-aminobutanoate degradation (1/3 steps found)
- superpathway of L-arginine and L-ornithine degradation (8/13 steps found)
- L-lysine degradation IV (2/5 steps found)
- superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (6/11 steps found)
- 4-aminobutanoate degradation V (3/7 steps found)
- L-lysine degradation X (2/6 steps found)
- L-lysine degradation III (1/6 steps found)
- L-lysine degradation I (1/7 steps found)
- nicotine degradation I (pyridine pathway) (4/17 steps found)
- superpathway of L-lysine degradation (8/43 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Butanoate metabolism
- Glutamate metabolism
- Lysine degradation
- Propanoate metabolism
- Valine, leucine and isoleucine degradation
- beta-Alanine metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.6.1.19
Use Curated BLAST to search for 2.6.1.19 or 2.6.1.22 or 2.6.1.48
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A1S8Y2 at UniProt or InterPro
Protein Sequence (425 amino acids)
>Sama_2636 gamma-aminobutyrate transaminase (EC 2.6.1.19) (Shewanella amazonensis SB2B) MSLTNDSLMVRRRAAVAGGVGQIHPVFTERAENATVWDVEGREYIDFAGGIAVLNTGHLH PKVKAAVAEQLEKFSHTCFMVLGYESYVAVCEKLNQLVPGDFAKKSALFTSGSEAVENAI KVARAYTKRAGVIAFTSGYHGRTMAALALTGKVAPYSKGMGLMQANVFRAEFPCALHGVS EDDAMASIERIFKNDAEPSDIAAIILEPVQGEGGFYAATPGFMKRLRELCDREGIMLIAD EVQTGAGRTGTFFAMEQMGVAADITTFAKSIAGGFPLSGITGRAEVMDAIGPGGLGGTYG GSPLACAAALAVIEVFEEEKLLERSNAIGQTIKSAIGELASRYPQIAEVRGLGSMIAIEL MENGKPAPEYCPQVLTEARNRGLILLSCGTYGNVLRILVPITAPDEQIQRGLEIMAECFE AVLGK