Protein Info for Sama_2453 in Shewanella amazonensis SB2B

Annotation: 1,4-alpha-glucan branching enzyme (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 841 PF22019: GlgB_N" amino acids 16 to 106 (91 residues), 61.4 bits, see alignment E=1.5e-20 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 116 to 731 (616 residues), 875.8 bits, see alignment E=8.7e-268 PF02922: CBM_48" amino acids 132 to 214 (83 residues), 73.8 bits, see alignment E=2.3e-24 PF00128: Alpha-amylase" amino acids 284 to 353 (70 residues), 32.3 bits, see alignment E=1.3e-11 PF02806: Alpha-amylase_C" amino acids 637 to 731 (95 residues), 87.4 bits, see alignment E=1.3e-28

Best Hits

Swiss-Prot: 65% identical to GLGB_SHEB9: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Shewanella baltica (strain OS195)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 100% identity to saz:Sama_2453)

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S8F1 at UniProt or InterPro

Protein Sequence (841 amino acids)

>Sama_2453 1,4-alpha-glucan branching enzyme (RefSeq) (Shewanella amazonensis SB2B)
MTSVQSQDSTLISEDAARALARGQYVDVFSLLGMHADNNQLVVRCFLPGALAVEVLSAKD
GKKVASLSAEAREGMFAGVMGRRVKPFAYLLRVAYPSHVEDVYDPYGFDSLLSDYDLYLF
GEGRQLYAQDFLGANPREVEGVAGVLFGVWAPNASRVSVVGDFNHWDGRVHVMRFHPASG
VWDIFIPGVHAHAHYKFEIMTADGELLIKSDPFAKTMEAAPNNASRVAPQSSFVWQDQDW
MQARNKDWHRQAMSIYELHLGSWRRKGEAGDTWLTYRELADELVPYVKSLGFTHIELMPL
SEFPFDGSWGYQPVGLFAPTQRFGSSDDLKYLIDACHRAGIGVLMDWVIAHFPADAHGLA
RFDGTCLYEHQDPRRGRHPDWDTLIFNYGRAEVQSFLLSAATVWLRDFHIDGLRLDAVSS
MLYLDYSRSEGEWLPNADGGRENHEAIECLKLINSRLYGEFPGIVMIAEESTAWDGVCRP
VSEGGLGFGFKWNMGWMNDTLAYLGKDPIHRRWHHHQLTFGIMYAHSEQFILSLSHDEVV
HGKASLLSKIPGDDWQKFATLRAYYGYMWGFPGKKLLFMGGEFGQRDEWNHDKSLDWHLL
QYAPHQGLSLWLADLNRLYQRHTVLGQTDHDLSRFCWLDCDNAEQGIYAFVRGEGDDALV
FVVNMTPQLHQGYRLGLPCEGDWRELLNSDASCYGGCNQGNGGVIVCTPEPAGGKAQSAL
VTIPPLGCLVLGLSRDKSPAISSESSADKALPVKKAPASTAPALIKAKPGTKTKPVTQTK
PVTKAKASTTDNAPAKAKSGTKPSTKPSTKPSTTPSTKPSAKSVAKAPSNAAKTKAEDPG
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