Protein Info for Sama_2451 in Shewanella amazonensis SB2B

Annotation: phosphorylase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 824 transmembrane" amino acids 437 to 456 (20 residues), see Phobius details TIGR02093: glycogen/starch/alpha-glucan phosphorylases" amino acids 39 to 821 (783 residues), 1137.7 bits, see alignment E=0 PF00343: Phosphorylase" amino acids 114 to 822 (709 residues), 1046 bits, see alignment E=0

Best Hits

Swiss-Prot: 52% identical to PHSG_SYNY3: Glycogen phosphorylase (glgP) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K00688, starch phosphorylase [EC: 2.4.1.1] (inferred from 100% identity to saz:Sama_2451)

MetaCyc: 48% identical to glycogen phosphorylase (Escherichia coli K-12 substr. MG1655)
Phosphorylase. [EC: 2.4.1.1]

Predicted SEED Role

"Glycogen phosphorylase (EC 2.4.1.1)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S8E9 at UniProt or InterPro

Protein Sequence (824 amino acids)

>Sama_2451 phosphorylase (RefSeq) (Shewanella amazonensis SB2B)
MATKRLKAAGPETDTGRPQPCEPCDALPACLERQLRYNLCREESRHQDLFHALAHGVKEQ
LLDSWRETRKRDAAGQQRQVAYLSLEFLMGRTLGNALLSLGISDDARKALLPYSVTLEDL
ESEEHDAGLGNGGLGRLAACFLDSCASMDIAATGYGIRYEYGMFAQKIVDGYQIERPDRW
LKEGNPWEVRLHGQAVMVPFFGHTQSYVDRDGRRHMQWVGTQDVVAVPHDMPVPGYRNGR
INTLRLWKAEATDDFDLEEFNQGDYAEAVARKNLAEQITMVLYPNDASENGKELRLRQQY
FLSSASLQDLLRRHCARFGDNFADFHRHNVIQLNDTHPAIAVPELMRLLLDVYCLEWDEA
WHITSHTMAYTNHTLLPEALECWSVRMLGHMLPRVLEIIYEINARYLDEAAHHWPGDGEM
LARMSIIEEGAEPRVRMAYLAIVASFSVNGVAALHTELLRRGLFKDFHALWPHKFNNKTN
GVTPRRWLMNANPRLSALIDRRLGSAWREDLSRLESLNAFTSDSDFIHAWREVKSVNKEA
LAALVERECHVQFDTAMMFDVQVKRIHEYKRQLLNILHVIHLYQRILSGDTHGMVPRCVL
IGGKAAPGYAMAKLIIKLAGNVAHMVNSDPHVTPWLRFAFLPNYNVSAMEVIAPGTDLSE
QISTAGKEASGTGNMKFMMNGALTIGTLDGANIEILEAVGPDNFFLFGLNADEVAALKPH
YRPDEYIRHSVALEGVIELIRSGHFSLQEPGIFDPIVSAIESPWDQWMTAADFESYLSAQ
HQAAALYANKDAWTLCSIRNTAASGRFSSDATIARYRDEIWTGA