Protein Info for Sama_2358 in Shewanella amazonensis SB2B

Name: guaA
Annotation: GMP synthase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00888: GMP synthase (glutamine-hydrolyzing), N-terminal domain
  • amino acids 9 to 204 (196 residues), 265.5 bits, E=2e-83
• PF00117: GATase
  • amino acids 10 to 200 (191 residues), 150.4 bits, E=1.2e-47
• PF07722: Peptidase_C26
  • amino acids 74 to 183 (110 residues), 38.2 bits, E=3.5e-13
• TIGR00884: GMP synthase (glutamine-hydrolyzing), C-terminal domain
  • amino acids 213 to 525 (313 residues), 515.1 bits, E=6.6e-159
• PF02540: NAD_synthase
  • amino acids 216 to 290 (75 residues), 27.9 bits, E=3.2e-10
• PF00958: GMP_synt_C
  • amino acids 433 to 524 (92 residues), 146 bits, E=6.9e-47

Best Hits

Swiss-Prot: 100% identical to GUAA_SHEAM: GMP synthase [glutamine-hydrolyzing] (guaA) from Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)

KEGG orthology group: K01951, GMP synthase (glutamine-hydrolysing) [EC: 6.3.5.2] (inferred from 100% identity to saz:Sama_2358)

MetaCyc: 84% identical to GMP synthetase (Escherichia coli K-12 substr. MG1655)
GMP synthase (glutamine-hydrolyzing). [EC: 6.3.5.2]; 6.3.5.2 [EC: 6.3.5.2]

Predicted SEED Role

No annotation

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (8/8 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• guanosine ribonucleotides de novo biosynthesis (4/4 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (1/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (1/4 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Drug metabolism - other enzymes
• Glutamate metabolism
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.2

Sequence Analysis Tools

See A1S856 at UniProt or InterPro

Protein Sequence (525 amino acids)

>Sama_2358 GMP synthase (RefSeq) (Shewanella amazonensis SB2B)
MSNIHEHKILILDFGSQYTQLIARRIREIGVYCELWAWDVTEAQIREFAPNGIVLAGGPE
SVTEDNSPRAPEYVFDAGVPVLGICYGMQTMSEQLGGKVIQGVGEREFGYAQVEVLTDSA
LLRHIEDGISDAGKPVLDVWMSHGDKVSAIPEGFVAVAKTETCPFAVMANEDKKFYGVQF
HPEVTHTRQGKRMLEHFVMDICGCDNKWQPASIIEDAVERIKAQVGDDEVILGLSGGVDS
SVTAMLLHRAIGERLTCVFVDNGLLRLNEAKQVLEMFGDHFGLNIVHVDAENRFLEALKG
ENDPEAKRKIIGRVFVEIFDEEASKCVNAKWLAQGTIYPDVIESAGSATGKAHVIKSHHN
VGGLPDDMELGLVEPLRELFKDEVRKIGLELGLPYDMLYRHPFPGPGLGVRVLGEVKKEY
CDLLRRADAIFIEELHRADLYNKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDF
MTAHWAHLPYDFLGRVSNRIINEVDGISRVVYDISGKPPATIEWE