Protein Info for Sama_2244 in Shewanella amazonensis SB2B

Annotation: polysaccharide biosynthesis protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 644 transmembrane" amino acids 20 to 38 (19 residues), see Phobius details amino acids 50 to 70 (21 residues), see Phobius details amino acids 82 to 105 (24 residues), see Phobius details amino acids 111 to 131 (21 residues), see Phobius details PF13727: CoA_binding_3" amino acids 69 to 237 (169 residues), 30.5 bits, see alignment E=1.3e-10 PF04321: RmlD_sub_bind" amino acids 283 to 462 (180 residues), 34.9 bits, see alignment E=3.3e-12 PF01370: Epimerase" amino acids 283 to 476 (194 residues), 60.9 bits, see alignment E=4.6e-20 PF02719: Polysacc_synt_2" amino acids 283 to 576 (294 residues), 417.8 bits, see alignment E=7.7e-129 PF01073: 3Beta_HSD" amino acids 284 to 416 (133 residues), 22.3 bits, see alignment E=2.1e-08 PF16363: GDP_Man_Dehyd" amino acids 284 to 411 (128 residues), 33.5 bits, see alignment E=1.1e-11

Best Hits

KEGG orthology group: None (inferred from 100% identity to saz:Sama_2244)

MetaCyc: 60% identical to UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining) (Shigella sonnei)
RXN-13252 [EC: 4.2.1.135]

Predicted SEED Role

"Nucleoside-diphosphate sugar epimerase/dehydratase"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.135

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S7U3 at UniProt or InterPro

Protein Sequence (644 amino acids)

>Sama_2244 polysaccharide biosynthesis protein (RefSeq) (Shewanella amazonensis SB2B)
MDFFQFLFSLKRPHKRMVSVAVDSVFLVLSFWFALFVRLDSVEVVLNSQFWLLIALVCPV
SIAAFVKLGLYRAVLRYLGTQAMTAIVLGVIVSTLAMVSFAYFLSVDLPRTVPFIFASFA
LVFVGGARALMRSLVGNGINRRGEPVIIYGAGVSGRQTALALAQSHEYHPFAFVDDDETL
HGTVIQGLHVHSPSIIKKLIKQQKASRVLLAMPSASRARRQQILQTLEPLSVKVMTLPAM
ADLVSGNKLFSDVKEVEIEDLLGRDAVAPRKDLMAANITGKVVMVTGAGGSIGSELCRQI
LRLAPRKLVLFELSEFGLYSIERELQAIRAELGLDVELFPMMGSVQRQNRVQAVMEAFKV
QTVYHAAAYKHVPLVEHNVVEGVRNNVFGTLYTAQAAIATGVETFVLISTDKAVRPTNVM
GTTKRMAELVLQALSKLNSGTRFCMVRFGNVLGSSGSVVPLFRKQIANGGPVTVTHPEIT
RFFMTIPEASQLVIQAGAMGIGGDVFVLDMGQSVKIVDLARKMIRLSGFEVKDEHNPDGD
IEIQFSGLRPGEKLYEELLIGDNVEGTGHERIMTAHEVFLTWEELKPVLDKLDMACHAFD
HESIRELMLKAPTGFNPTDGICDLVWLNRRHVEDRSNVVALKGQ