Protein Info for Sama_2056 in Shewanella amazonensis SB2B

Annotation: ATP-dependent Clp protease, ATP-binding subunit ClpA (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 753 TIGR02639: ATP-dependent Clp protease ATP-binding subunit ClpA" amino acids 3 to 736 (734 residues), 1095 bits, see alignment E=0 PF02861: Clp_N" amino acids 13 to 62 (50 residues), 50.3 bits, see alignment 1.4e-16 PF00004: AAA" amino acids 213 to 318 (106 residues), 45.6 bits, see alignment E=6.2e-15 amino acids 494 to 609 (116 residues), 38 bits, see alignment E=1.4e-12 PF17871: AAA_lid_9" amino acids 352 to 455 (104 residues), 94.3 bits, see alignment E=2.7e-30 PF07724: AAA_2" amino acids 488 to 649 (162 residues), 203 bits, see alignment E=2.2e-63 PF00158: Sigma54_activat" amino acids 492 to 617 (126 residues), 22 bits, see alignment E=7.7e-08 PF07728: AAA_5" amino acids 493 to 609 (117 residues), 44.8 bits, see alignment E=8.6e-15 PF10431: ClpB_D2-small" amino acids 656 to 734 (79 residues), 79.8 bits, see alignment E=7.9e-26

Best Hits

Swiss-Prot: 70% identical to CLPA_ECO57: ATP-dependent Clp protease ATP-binding subunit ClpA (clpA) from Escherichia coli O157:H7

KEGG orthology group: K03694, ATP-dependent Clp protease ATP-binding subunit ClpA (inferred from 100% identity to saz:Sama_2056)

MetaCyc: 70% identical to ATP-dependent Clp protease ATP-binding subunit ClpA (Escherichia coli K-12 substr. MG1655)
Endopeptidase Clp. [EC: 3.4.21.92]

Predicted SEED Role

"ATP-dependent Clp protease ATP-binding subunit ClpA" in subsystem Proteolysis in bacteria, ATP-dependent

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.92

Use Curated BLAST to search for 3.4.21.92

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S7A5 at UniProt or InterPro

Protein Sequence (753 amino acids)

>Sama_2056 ATP-dependent Clp protease, ATP-binding subunit ClpA (RefSeq) (Shewanella amazonensis SB2B)
MLNKDLEVTLNLAFQQARDARHEYMTVEHLLLALIDNPSAQDALVACGANIDKLREEVAT
FISQTTPVITDPDDDRETQPTLGFQRVLQRAVFHVQSSGRNEVTGANVLVAIFSEQESQA
VYLLRRSDITRLDVVNYISHGFSKSEDAPQESEQERIEDHSEQGEEQRSMLSQFAANLNL
LAAQGVIDPLIGRDNEIERSIQILCRRRKNNPLLVGEAGVGKTAIAEGLAYRIVNDDVPE
VMRDATVYSLDLGALLAGTKYRGDFEKRFKSLLKELTADKHAILFIDEIHTIIGAGAASG
GVMDASNLLKPLLSSGTLRCMGSTTFQEYQSIFEKDRALARRFQKIDINEPSVAETTKIL
MGLKSKYEEHHGVRYTQAALACAAELSAKHINDRHLPDKAIDVIDEAGARMAMQPASKRR
KTIGQAEIEAIIAKIARIPEKSVSTSDKDMLKNLERNLKMVVFGQDKAIESLSAAIRLSR
SGLGADNKPVGSFLFAGPTGVGKTEVTNQLAKCLGLKLVRFDMSEYMEAHTVSRLIGAPP
GYVGYDQGGLLTDAVIKQPHCVVLLDEIEKAHPDVYNLLLQVMDHGTLTDNNGRKADFRH
VTLVMTTNAGVQETVRKSIGFKQQDHSHDAMSEINRVFSPEFRNRLDSIIWFNHLDLTII
AKVVDKFLTELQAQLDAKRVTLMVSDEARTLLAEKGYDKAMGARPMARVVKDLIKRPLAD
EILFGELEHGGMAHVDAKDGEIVISAERLEKVT