Protein Info for Sama_1731 in Shewanella amazonensis SB2B

Annotation: DNA gyrase subunit A (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 901 TIGR01063: DNA gyrase, A subunit" amino acids 8 to 863 (856 residues), 1228.2 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 32 to 506 (475 residues), 557.2 bits, see alignment E=2.7e-171 PF03989: DNA_gyraseA_C" amino acids 537 to 584 (48 residues), 37.6 bits, see alignment 1.2e-13 amino acids 587 to 636 (50 residues), 36.9 bits, see alignment 1.9e-13 amino acids 644 to 687 (44 residues), 40.5 bits, see alignment 1.5e-14 amino acids 692 to 708 (17 residues), 10.6 bits, see alignment (E = 3.1e-05) amino acids 734 to 761 (28 residues), 25.2 bits, see alignment (E = 8.7e-10) amino acids 768 to 812 (45 residues), 38.8 bits, see alignment 5.1e-14 amino acids 817 to 863 (47 residues), 41.2 bits, see alignment 8.9e-15

Best Hits

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S6D0 at UniProt or InterPro

Protein Sequence (901 amino acids)

>Sama_1731 DNA gyrase subunit A (RefSeq) (Shewanella amazonensis SB2B)
MTDLASSISPINIEDELKNSYLDYAMSVIVGRALPDVRDGLKPVHRRVLFAMNELKNDWN
KPYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYTLVDGQGNFGSIDGDAAAAM
RYTEIRMEKLAHELLADLEKETVDFVPNYDGTEQIPAVLPTKIPNLLVNGASGIAVGMAT
NIPPHNLTEVVKGCLALIEEPELSIEQLMEYIPGPDFPTAAIINGKKGIEEAYRTGRGKA
IMRARAEVEIEDNGRERIIVTEIPYQVNKARLIEKIAELVKDKKIEGISGLRDESDKDGM
RIVIEIKRGEVGEVVLNNLYAQTQMQSSFGINMVALANGQPKLFNLKEMLEAFILHRREV
VTRRTVYELRKARDRAHILEALAVALANIDPIIALIKASASPSDAKAGLIAKGWELGTVK
AMLEKAGDDAARPEWLEPEFGIRDGLYFMTEPQAQAILDLRLHKLTGLEHEKILQEYEEL
LEIIAGLLHILRSPERLMEVIKEELELILEQFGDNRRTEINASAVDISLEDLINEEDVVV
TLSHLGYAKYQPLTDYQAQRRGGKGKAATKVKDEDFVEKLLVANTHDTILCFSDFGKLYW
LKVYQLPLASRQARGRPIVNLLPLQEGERITAILPVREYEEGKYILMATSHGTVKKTALT
EYSRPRSNGIIAVNLKDGDQLIGVDITDGSSEIMLFSDAGKVVRFKEGEEVAVVDENGNP
VLDEEGKPQINFKGVRPMGRGATGVRGIALEEGQRVVSLIVPKDDSAILTVTANGFGKRT
ELAEYPAKSRATKGVVSIKVTERNGPVVGAVQVGENDEIMLISDKGTLVRTPAIGVSIIG
RNTQGVTIIRTADDETVVGLQRIDEIQDDGSEYLEEGVEGAEGDIIAEADTQPTDDANDE
A