Protein Info for Sama_1729 in Shewanella amazonensis SB2B

Annotation: phosphoglycolate phosphatase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 213 PF00702: Hydrolase" amino acids 3 to 177 (175 residues), 90.6 bits, see alignment E=3.7e-29 TIGR01449: phosphoglycolate phosphatase, bacterial" amino acids 4 to 200 (197 residues), 173.3 bits, see alignment E=7.5e-55 PF13419: HAD_2" amino acids 4 to 181 (178 residues), 116.1 bits, see alignment E=4.1e-37 PF12710: HAD" amino acids 4 to 174 (171 residues), 41.9 bits, see alignment E=3.1e-14 TIGR01549: HAD hydrolase, family IA, variant 1" amino acids 98 to 177 (80 residues), 33.6 bits, see alignment E=7.2e-12 PF13242: Hydrolase_like" amino acids 138 to 187 (50 residues), 25.8 bits, see alignment E=1.7e-09

Best Hits

Swiss-Prot: 40% identical to GPH_XYLFT: Phosphoglycolate phosphatase (gph) from Xylella fastidiosa (strain Temecula1 / ATCC 700964)

KEGG orthology group: K01091, phosphoglycolate phosphatase [EC: 3.1.3.18] (inferred from 100% identity to saz:Sama_1729)

Predicted SEED Role

"Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase" in subsystem 2-phosphoglycolate salvage

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.18

Use Curated BLAST to search for 3.1.3.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S6C8 at UniProt or InterPro

Protein Sequence (213 amino acids)

>Sama_1729 phosphoglycolate phosphatase (RefSeq) (Shewanella amazonensis SB2B)
MGGVLFDLDGTLVDTAPDLVAALNLALADFGFSRADLDSMRPVASHGSLALVKAAKPDLD
EDVAIQIQQALLAHYGKVNGTEAKLFPGINLLLEHLESRHIPWGIVTNKAARYARPLLDR
LGLAHQCSTLISGDTVTRGKPDAAPMLLGASHLGRHTEHLLYLGDAKRDMEAARNAGMVS
VLASWGYLSSLDEAHDWPIDIHLKRPQDLIAHL