Protein Info for Sama_1650 in Shewanella amazonensis SB2B

Annotation: phosphotransferase system, enzyme I (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 567 PF05524: PEP-utilisers_N" amino acids 4 to 125 (122 residues), 64.1 bits, see alignment E=2.2e-21 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 4 to 560 (557 residues), 467.4 bits, see alignment E=3e-144 PF00391: PEP-utilizers" amino acids 154 to 224 (71 residues), 65.4 bits, see alignment E=4.2e-22 PF02896: PEP-utilizers_C" amino acids 250 to 538 (289 residues), 324.9 bits, see alignment E=6.7e-101

Best Hits

Swiss-Prot: 38% identical to PT1_HAEIN: Phosphoenolpyruvate-protein phosphotransferase (ptsI) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] (inferred from 100% identity to saz:Sama_1650)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.9

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S650 at UniProt or InterPro

Protein Sequence (567 amino acids)

>Sama_1650 phosphotransferase system, enzyme I (RefSeq) (Shewanella amazonensis SB2B)
MQVNGIAIKPGVTFGEALHFNTGHKPLDYRILPIKRIPQQLSRLDGGLARLKQQLSASLQ
ALDSQSDAYALVEADLLYLDDPDLRAHIADTITQLQFSACVSIERVFAHQASELEALDDP
YLAQRAEDVRSLGKRLIQAVFGHPGQEPGKLKVPTILLADDISPAEFAVLPLENVAGIVL
KSGGLTSHTAILARAAGIPALLSCPYSELGINNGDQLAIDGDAGALYRNPEGGTLELLRQ
HEASARLAREQLDQYRDKPAMTQDGHEISLLANVGNLNDVLKVSGLGADGIGLLRTEFML
MHSATLPDERAQYQLYSDAIHALEGRVLTIRTLDIGADKELPCLCQVKEENPALGLRGIR
YTLANPQLLKTQLRAVLRAANHGHVRLMFPMVNQVEELDAVLALLETCRRELDDEEKGFG
DISLGIVVETPAAVLNLPAMLPMLDFVSIGTNDLTQYAMAADRGNPCLARDYPALSPAVL
RLISMTLQSARSQDVKVSLCGEMGSDPRLVPLLIGLGFDELSVNVGAMLEVKAAICRQEF
DRCTQLAGRALMADRLSELDACISSYK