Protein Info for Sama_1586 in Shewanella amazonensis SB2B

Name: pepN
Annotation: aminopeptidase N (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 852 TIGR02414: aminopeptidase N" amino acids 11 to 850 (840 residues), 1150.4 bits, see alignment E=0 PF17900: Peptidase_M1_N" amino acids 82 to 183 (102 residues), 54.9 bits, see alignment E=2.6e-18 PF01433: Peptidase_M1" amino acids 223 to 433 (211 residues), 190.3 bits, see alignment E=7.2e-60 PF11940: DUF3458" amino acids 441 to 533 (93 residues), 99.1 bits, see alignment E=3.1e-32 PF17432: DUF3458_C" amino acids 537 to 850 (314 residues), 353.6 bits, see alignment E=2.4e-109

Best Hits

Swiss-Prot: 56% identical to AMPN_ECOLI: Aminopeptidase N (pepN) from Escherichia coli (strain K12)

KEGG orthology group: K01256, aminopeptidase N [EC: 3.4.11.2] (inferred from 100% identity to saz:Sama_1586)

MetaCyc: 56% identical to aminopeptidase N (Escherichia coli K-12 substr. MG1655)
Cytosol nonspecific dipeptidase. [EC: 3.4.13.18]; Cytosol alanyl aminopeptidase. [EC: 3.4.13.18, 3.4.11.14, 3.4.11.2]; 3.4.11.- [EC: 3.4.13.18, 3.4.11.14, 3.4.11.2]

Predicted SEED Role

"Membrane alanine aminopeptidase N (EC 3.4.11.2)" (EC 3.4.11.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.2

Use Curated BLAST to search for 3.4.11.14 or 3.4.11.2 or 3.4.13.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S5Y7 at UniProt or InterPro

Protein Sequence (852 amino acids)

>Sama_1586 aminopeptidase N (RefSeq) (Shewanella amazonensis SB2B)
MSELQAKHLKDYQSPAFTISELDMLVDLYPSKTRVIARSQVIRCRDNEALLLDGEGLELK
SVKVNNEAYPYQIVAGQLRLEGLPAHFALEIETEIDPVGNSSLEGLYMSDGAYCTQCEAE
GFRRITYFPDRPDVLAKYRVRIEAPKTGFPYLLSNGNKVEAGALDDDRHYVVWEDPFPKP
SYLFALVAGDFDLLQDSFVTQSGRHVALEVFVDKGNLHKAHHAMASLKKSMAWDESRFGL
EYDLDIYMIVAVDFFNMGAMENKGLNIFNTKYVLADIESATDDDFHGIESVVGHEYFHNW
TGNRVTCRDWFQLSLKEGLTVFRDQEFSSDVGSRAVNRIQAIKVIKNQQFAEDSGPMAHP
IRPQSVIEMNNFYTVTVYNKGAEVIRMMHTLLGEAGFQAGMKLYFQRHDGQAVTCDDFVG
AMEDASGVDLSQFRLWYNQAGTPTLKVSDSFDARSGEYRLVVLQQLPPQAGDDAKPMHIP
FDLELIGDDGKSLLNKVLDVKEAHNEFVFEGLSTRPVPSLLQNFSAPVKLEYDFTTAQLI
SLMRNASSEVARWEASVSLISRAIWGNVNALVSGGDMALDTRVADAFRAVLLDEELDPQL
AAEILNIPSINALIEQVDSVNFDALVSARAFVLEELSANLEDELLVRYRELYHIDSAPAR
ALKNACLAMLARGGLCPEELVEKQFNESCNMTDSLAALGAAMAGELDCQDSLLERFEQRW
VDTPLVMDKWFMLQASRQDDAVLGRIDALVSHKAFSLNNPNRVRSLIGCFAANNPVQFHR
EDGEGYRLLTRYLKQLNGVNPQVAARIITPLIQFGKFDEGRKQNMKACLEELIGMPELSR
DLYEKVSRALSQ