Protein Info for Sama_1498 in Shewanella amazonensis SB2B

Annotation: formate C-acetyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01255: formate acetyltransferase
  • amino acids 11 to 759 (749 residues), 1334.3 bits, E=0
• PF02901: PFL-like
  • amino acids 25 to 614 (590 residues), 720.8 bits, E=1.9e-220
• PF01228: Gly_radical
  • amino acids 630 to 741 (112 residues), 122.2 bits, E=1.4e-39

Best Hits

Swiss-Prot: 80% identical to PFLB_ECOLI: Formate acetyltransferase 1 (pflB) from Escherichia coli (strain K12)

KEGG orthology group: K00656, formate C-acetyltransferase [EC: 2.3.1.54] (inferred from 80% identity to ecg:E2348C_0896)

MetaCyc: 80% identical to pyruvate formate-lyase (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Pyruvate formate-lyase (EC 2.3.1.54)" in subsystem Butanol Biosynthesis or Fermentations: Mixed acid (EC 2.3.1.54)

MetaCyc Pathways

• mixed acid fermentation (15/16 steps found)
• superpathway of L-threonine metabolism (15/18 steps found)
• L-threonine degradation I (6/6 steps found)
• pyruvate fermentation to acetate IV (3/3 steps found)
• pyruvate fermentation to ethanol I (3/3 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (7/9 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
• superpathway of N-acetylneuraminate degradation (16/22 steps found)
• reductive monocarboxylic acid cycle (1/2 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Propanoate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.54

Sequence Analysis Tools

See A1S5P9 at UniProt or InterPro

Protein Sequence (760 amino acids)

>Sama_1498 formate C-acetyltransferase (RefSeq) (Shewanella amazonensis SB2B)
MTDKTELFQTAWEGFVPGDWKSDVNVRDFIQTNYTPYEGDDSFLAGPTAATNALWAKVME
GIKEENRTHAPLDFDTDKVSTITSHDAGYIEKELETIVGLQTDAPLKRAMLPNGGIRMVE
SSCAAYNRELNADIKYVYSELRKTHNQGVFDIYTPEILACRKSGVLTGLPDAYGRGRIIG
DYRRVALYGIDYLMADKLKQFTSLQSRFEEGEDLQATMQLREEIAEQHRALAQMKKMAAK
YGYDISGPAKNAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSSFIDIYIERDLKNGTLTEE
QAQEMIDHFVMKLRMVRFLRTPEYDELFSGDPIWATESIGGMGLDGRTLVTKSSFRFLHT
LYNMGPSPEPNITVLWSEKLPENFKTFCAKVSIDTSSIQYENDDLMRPDFESDDYAIACC
VSPMVVGKHMQFFGARANLAKTMLYAINGGVDEKLKVQIGPKSEPITSEYLDYDDVMSRL
DNLMEWLATQYVSALNAIHFMHDKYSYEAALMALHDRDVRRTMACGIAGLSIAADSLSAI
KYAKVKPVRDEHGIAVDFEIEGDYPKFGNNDARVDDIACMLVEGFMAKIRNKKMYRNAIP
TQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPMHGRDEKGAIASLTSVAKLPFAHA
QDGISYTFSIVPNALGKDEKARERNLAALMDGYFRHSEGREGGQHLNVNVMNREMLEDAI
ANPDKYPQLTIRVSGYAVRFNSLTPEQQQDVITRTFTQGL