Protein Info for Sama_1443 in Shewanella amazonensis SB2B

Annotation: heat shock protein HtpX (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 transmembrane" amino acids 14 to 36 (23 residues), see Phobius details amino acids 42 to 63 (22 residues), see Phobius details amino acids 164 to 187 (24 residues), see Phobius details amino acids 200 to 224 (25 residues), see Phobius details PF01435: Peptidase_M48" amino acids 83 to 295 (213 residues), 134.6 bits, see alignment E=1.8e-43

Best Hits

Swiss-Prot: 86% identical to HTPX_SHESA: Protease HtpX (htpX) from Shewanella sp. (strain ANA-3)

KEGG orthology group: K03799, heat shock protein HtpX [EC: 3.4.24.-] (inferred from 100% identity to saz:Sama_1443)

Predicted SEED Role

"Probable protease htpX homolog (EC 3.4.24.-)" (EC 3.4.24.-)

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S5J4 at UniProt or InterPro

Protein Sequence (296 amino acids)

>Sama_1443 heat shock protein HtpX (RefSeq) (Shewanella amazonensis SB2B)
MGDLPDKGAEMKRVFLFLLTNLAVLLVASIVMSILGVDTRSMSGLLVFAAIFGFGGAFFS
LAISKWMAKKSMGCEVITTPRDATEKWLIDTVARQAQQAGIKMPEVAIYNSPEMNAFATG
PSKNNSLVAVSTGLLYGMTQDEVEGVLAHEVSHVANGDMVTLTLIQGVVNTFVIFAARVV
AGFINNFLSSDEEGEGLGTFAYIAVVFVLEMLFGILASIVVAYFSRIREYRADEGGAKLA
GRHKMVAALERLRNGPETGAMPAQMAAFGINGKRSLGELLMSHPPLEKRIQALKAQ