Protein Info for Sama_1427 in Shewanella amazonensis SB2B

Name: sucA
Annotation: 2-oxoglutarate dehydrogenase E1 component (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF16078: 2-oxogl_dehyd_N
  • amino acids 12 to 50 (39 residues), 66.3 bits, see alignment (E = 2.8e-22)
• TIGR00239: oxoglutarate dehydrogenase (succinyl-transferring), E1 component
  • amino acids 16 to 936 (921 residues), 1399.9 bits, E=0
• PF00676: E1_dh
  • amino acids 232 to 519 (288 residues), 166.6 bits, E=1.3e-52
• PF02779: Transket_pyr
  • amino acids 598 to 790 (193 residues), 166.5 bits, E=9.8e-53
• PF16870: OxoGdeHyase_C
  • amino acids 795 to 937 (143 residues), 172.5 bits, E=1e-54

Best Hits

Swiss-Prot: 70% identical to ODO1_ECO57: 2-oxoglutarate dehydrogenase E1 component (sucA) from Escherichia coli O157:H7

KEGG orthology group: K00164, 2-oxoglutarate dehydrogenase E1 component [EC: 1.2.4.2] (inferred from 72% identity to avr:B565_1675)

MetaCyc: 70% identical to subunit of E1(0) component of 2-oxoglutarate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Oxoglutarate dehydrogenase (succinyl-transferring). [EC: 1.2.1.105, 1.2.4.2]

Predicted SEED Role

"2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2)" in subsystem TCA Cycle (EC 1.2.4.2)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• TCA cycle III (animals) (9/10 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• 2-oxoglutarate decarboxylation to succinyl-CoA (3/3 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• TCA cycle VIII (Chlamydia) (5/6 steps found)
• L-tryptophan degradation III (eukaryotic) (4/15 steps found)
• L-tryptophan degradation XI (mammalian, via kynurenine) (5/23 steps found)
• superpathway of L-lysine degradation (8/43 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Lysine degradation
• Tryptophan metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.105 or 1.2.4.2

Sequence Analysis Tools

See A1S5H8 at UniProt or InterPro

Protein Sequence (940 amino acids)

>Sama_1427 2-oxoglutarate dehydrogenase E1 component (RefSeq) (Shewanella amazonensis SB2B)
MHQGSMKAWLESSHLYGSNATYVEEMYEAYQEDPTSVSDDWRAVFDNLPPVNGASADVPE
ASHSKIRDYFRSLAQEGQRKGAGRVTDPEVDAKQVKVLQLINAYRFRGHQNANLDPLELW
KRDAVVELDPAFHGLTADDMQREFNTGSFAYGGETLKLGELVKALKATYCGSIGAEYMHI
TDTDEKRWIQQRLEPSMGRAAFDKGVKTRILEGLNAAEGMEKYLGAKFPGAKRFSLEGGD
ALVPMMREIIYRAGEAGTKEIVVGMAHRGRLNVLVNVLGKRPAELFDEFAGKHAESSGSG
DVKYHQGFSSDFETPGGNVHLALAFNPSHLEIVNPVVMGSVRARQDRRGCKDGLSVMPIT
IHGDSAIAGQGIVQETFNMSQTRGFRVGGSIRIVVNNQVGFTTSNHHDVRSTEYCTDIAK
MVQAPIFHVNADDPEAVAFVAQVAVDYRNAFKRDVVIDLVCYRRHGHNEADEPSATQPLM
YQKIKKHPTPRKIYADRLIAENAVSADDVTAMINGYRDALDQGDCVVKEWRPMSLRTVDW
SPFIGQDWDVSYEPTVALARLQKLAEKLAYVPETHPLQSRVAKIYADRVAMAKGEKLLDW
GFAETLAYATILEDNNRVRITGQDSGRGTFFHRHAVLHNQNDATTYMPLRNLAQEQGPVD
ITDSVLSEASVLAFEYGYATAEPGGLTIWEAQFGDFANCAQVVIDQFLSSGEQKWGRLCG
LTMLLPHGYEGQGPEHSSARLERFLQLCANHNMQVCVPSTPAQVYHMLRRQVVRPLRRPL
VVMSPKSLLRHPLAVSSLEELANGTFQNVIGEIDSLDPKAVNRVVFCSGKVYFELLERRR
KENLTNVAIIRVEQLYPFPAEEMAAILAEYQHVTDFVWCQEEPQNQGAWYCSQHHFWAAI
PKGATLTYAGREAAAAPACGYPELHAHQQESLVVDALNLK