Protein Info for Sama_1321 in Shewanella amazonensis SB2B

Updated annotation (from data): Succinylarginine dihydrolase (EC 3.5.3.23)
Rationale: Specifically important for utilizing L-Arginine. Automated validation from mutant phenotype: the predicted function (SUCCARGDIHYDRO-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: succinylarginine dihydrolase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04996: AstB
  • amino acids 3 to 443 (441 residues), 752.3 bits, E=8.1e-231
• TIGR03241: succinylarginine dihydrolase
  • amino acids 4 to 443 (440 residues), 755.7 bits, E=9.4e-232

Best Hits

Swiss-Prot: 100% identical to ASTB_SHEAM: N-succinylarginine dihydrolase (astB) from Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)

KEGG orthology group: K01484, succinylarginine dihydrolase [EC: 3.5.3.23] (inferred from 100% identity to saz:Sama_1321)

MetaCyc: 57% identical to N-succinylarginine dihydrolase (Escherichia coli K-12 substr. MG1655)
N-succinylarginine dihydrolase. [EC: 3.5.3.23]

Predicted SEED Role

"Succinylarginine dihydrolase (EC 3.5.3.23)" in subsystem Arginine and Ornithine Degradation (EC 3.5.3.23)

MetaCyc Pathways

• L-arginine degradation II (AST pathway) (5/5 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.3.23

Sequence Analysis Tools

See A1S572 at UniProt or InterPro

Protein Sequence (444 amino acids)

>Sama_1321 Succinylarginine dihydrolase (EC 3.5.3.23) (Shewanella amazonensis SB2B)
MKHFEANFDGLVGPTHNYAGLSFGNVASQSNAAQVSNPKDAAKQGLKKAKALADMGMVQG
MLAPQERPDIHTLRRVGFTGSDADVLSQAAKASPVLLQACASASSMWTANAATVSPSADS
DDGKLHFTPANLVDKLHRSIEPVTTGNILKAIFTDERYFAHHQHLPEHPHFGDEGAANHT
RLCHDYGQAGVEVFVYGRSVADLSRPAPVKYPARQTLEASQAVARLHQLSDDRTVYMQQN
PDVIDQGVFHNDVIAVGNQNVLFYHEQAFLETQAKLAEIDKKMHGNMYFIEVPTAKVSVQ
DAVKSYLFNTQIITLSDGNMAIIAPTDCQENPAVHAYLNELVTLNTPIKAVHYFDVKQSM
QNGGGPACLRLRVAMNETELAAVNPQVMMNDALFARLNQWVDKHYRDRLSTQDLADPQLL
MESRTALDELTQIMKLGSVYQFQR