Protein Info for Sama_1250 in Shewanella amazonensis SB2B
Annotation: DNA polymerase II (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K02336, DNA polymerase II [EC: 2.7.7.7] (inferred from 100% identity to saz:Sama_1250)Predicted SEED Role
"DNA polymerase II (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.7.7.7
Use Curated BLAST to search for 2.7.7.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A1S501 at UniProt or InterPro
Protein Sequence (801 amino acids)
>Sama_1250 DNA polymerase II (RefSeq) (Shewanella amazonensis SB2B) MELSSPCYPGFNESLAPILLRGRVLTRANVQRSGDTVLEYLIKTVNGPVKVEVHNQRPMA FCHSDDVERLLVEGAETEVWSHPLELQSFSRQPVHAIYCRTGLMLKRLVNRAQELGIALY EADIKPEQRFLIERFVALDAEFFGHFDSSGPSRFVASRVRATSQHIPLKAVSLDIECSMS GELYSIGLYGDCEPKVIIVGEGETELTCHRDGVEHKVAVEWACDEVALLLSLQRWFMEVD PDILLGWAVVTFDLSLLWRRAKHHGMRLCIGRFGKPLAWKVEDKHRPETLDLSGRVVLDG IDWLKAAFYQFDSFALDRVAGELLGEGKAIHNPDDRGEEITRLFREDKAALAYYNLTDCR LVWDIFQKTDLIHFALERARLTGLEFGRVGASIAAFNHLYLPHLHRAGFVAPAMQTVPGP DSPGGYVMDSVPGLYRNVLVLDYKSLYPSIIRTFLIDPKGLVLGLSEPEAMSVEGFRGAR FSRQSPILPKLVATLASRREEAKANQNGPMSQAVKIIMNSLYGVLGSRGCVFHDYRLASS ITLRGHEIMRTTKAWIEAEGFQVIYGDTDSTFVWLGDEAGNAKATGRALVDMVNQRWREQ LREDKGLECFLELEFERHFEQFFMPTLRHSTEGSKKRYVGLYDNGNEKVLVFKGMEQVRS DWSPLARRVQYELYRRLFQQENLTEYVQEVEKSLYSGELDDELVFHKRLKRDISQYTAKS APHVKAAALLVSATGDANRGKRGAAIRYLFTKAGAEPLGFQSQPIDYDYYYDRQLLPILE PILAVLKINYQKSGAEQLLLI