Protein Info for Sama_1250 in Shewanella amazonensis SB2B

Annotation: DNA polymerase II (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 801 PF03104: DNA_pol_B_exo1" amino acids 192 to 319 (128 residues), 39.9 bits, see alignment E=5.1e-14 PF00136: DNA_pol_B" amino acids 401 to 783 (383 residues), 138.3 bits, see alignment E=5.2e-44

Best Hits

KEGG orthology group: K02336, DNA polymerase II [EC: 2.7.7.7] (inferred from 100% identity to saz:Sama_1250)

Predicted SEED Role

"DNA polymerase II (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S501 at UniProt or InterPro

Protein Sequence (801 amino acids)

>Sama_1250 DNA polymerase II (RefSeq) (Shewanella amazonensis SB2B)
MELSSPCYPGFNESLAPILLRGRVLTRANVQRSGDTVLEYLIKTVNGPVKVEVHNQRPMA
FCHSDDVERLLVEGAETEVWSHPLELQSFSRQPVHAIYCRTGLMLKRLVNRAQELGIALY
EADIKPEQRFLIERFVALDAEFFGHFDSSGPSRFVASRVRATSQHIPLKAVSLDIECSMS
GELYSIGLYGDCEPKVIIVGEGETELTCHRDGVEHKVAVEWACDEVALLLSLQRWFMEVD
PDILLGWAVVTFDLSLLWRRAKHHGMRLCIGRFGKPLAWKVEDKHRPETLDLSGRVVLDG
IDWLKAAFYQFDSFALDRVAGELLGEGKAIHNPDDRGEEITRLFREDKAALAYYNLTDCR
LVWDIFQKTDLIHFALERARLTGLEFGRVGASIAAFNHLYLPHLHRAGFVAPAMQTVPGP
DSPGGYVMDSVPGLYRNVLVLDYKSLYPSIIRTFLIDPKGLVLGLSEPEAMSVEGFRGAR
FSRQSPILPKLVATLASRREEAKANQNGPMSQAVKIIMNSLYGVLGSRGCVFHDYRLASS
ITLRGHEIMRTTKAWIEAEGFQVIYGDTDSTFVWLGDEAGNAKATGRALVDMVNQRWREQ
LREDKGLECFLELEFERHFEQFFMPTLRHSTEGSKKRYVGLYDNGNEKVLVFKGMEQVRS
DWSPLARRVQYELYRRLFQQENLTEYVQEVEKSLYSGELDDELVFHKRLKRDISQYTAKS
APHVKAAALLVSATGDANRGKRGAAIRYLFTKAGAEPLGFQSQPIDYDYYYDRQLLPILE
PILAVLKINYQKSGAEQLLLI