Protein Info for Sama_1057 in Shewanella amazonensis SB2B

Annotation: M16 family peptidase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 945 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF00675: Peptidase_M16" amino acids 59 to 183 (125 residues), 67 bits, see alignment E=1.8e-22 amino acids 532 to 654 (123 residues), 47.8 bits, see alignment E=1.5e-16 PF05193: Peptidase_M16_C" amino acids 218 to 391 (174 residues), 105.9 bits, see alignment E=2.4e-34 amino acids 682 to 857 (176 residues), 67.8 bits, see alignment E=1.2e-22

Best Hits

KEGG orthology group: K07263, zinc protease [EC: 3.4.24.-] (inferred from 100% identity to saz:Sama_1057)

Predicted SEED Role

"Protease, insulinase family/protease, insulinase family"

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S4F8 at UniProt or InterPro

Protein Sequence (945 amino acids)

>Sama_1057 M16 family peptidase (RefSeq) (Shewanella amazonensis SB2B)
MKKWILAAAISAALAGCAQHSSDSLALPEGVTLVEIVAADSAGVGIPYKKFQLANGLTVI
LHQDSSDPLVHVDVTYHVGSGRELPGRSGFAHLFEHMMFQGSENVADEQHFKVVTESGGT
LNGTTNTDRTNYFETVPSNQLEKMLWLESDRMGFFLPALTEEKFEVQRETVKNERAQRID
NQPYGRMYERFSQAMYPAGHPYSWPVIGWPEDLNRAELADVKQFFQRWYGPNNATLTIGG
DFDELQTLAWVNKYFGDIPRGPEVEALPKNQVTLDKDRYLSMEDKVHLPLIRMALPTVYA
SHQDEAALDLLANILGGGKTSLVYKNLVKEGYAVQASASHPCRELSCEMSFFALANPAKG
GSLKELEGKVRASIAEFERRGVTDEDLEKVKVQFEADTIFGLQSVRGKVSALAANETFYG
NPNMIAKDLQRYASVTKADVIRVFNQYVKDKPMVIMSVVPEGAKALVAAADNFTAPAPLL
AANAVAGELKVSEAKSSFDRSVMPQSGKAPVLKVPTLWRDTLGNGIAVLGTQSAETPTTE
IVIYLAGGHRLADVSKAGVAQLTAAMLNESTSQRSSEELTQALELLGASVQFSASDYQSE
IKLSALTEKLDDTLAVLKEKLLMPGFKAEDFERVKQQTLQGLKHSQSNPNYLASTGFAKL
LYGDNNALGVDATGTVDTVAALTLDDIKAFYQTQYKAGNAQMVAVSSAPKAALLSSLKTL
DSWQGEATAMPALGQLPGLSGGTIYVLDKPAAAQSVISIGKLALPFDAIGEYFKAGLMNY
PLGGAFNSRINLNLREDKGYTYGARSRFSGGSEVGQFLATASVRSDVTAAAVSEFIKEIT
TYNATGIRDDELNFMRNSISQGQALDYETPYQKAGFMRMIQRYNLNDRFTAEQDKIIAGI
QKDEIDALAKKHLNINEMVIFVVGDKASILPELEALGYPVKDLVL