Protein Info for Sama_0878 in Shewanella amazonensis SB2B

Annotation: GTP-binding protein LepA (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01393: elongation factor 4
  • amino acids 2 to 594 (593 residues), 984.9 bits, E=1.3e-300
• PF00009: GTP_EFTU
  • amino acids 2 to 182 (181 residues), 187.4 bits, E=4.2e-59
• TIGR00231: small GTP-binding protein domain
  • amino acids 6 to 176 (171 residues), 61.2 bits, E=1.1e-20
• PF03144: GTP_EFTU_D2
  • amino acids 205 to 275 (71 residues), 41.1 bits, E=3.9e-14
• PF00679: EFG_C
  • amino acids 399 to 483 (85 residues), 70.3 bits, E=2.4e-23
• PF06421: LepA_C
  • amino acids 487 to 593 (107 residues), 165.4 bits, E=7.7e-53

Best Hits

Swiss-Prot: 100% identical to LEPA_SHEAM: Elongation factor 4 (lepA) from Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)

KEGG orthology group: K03596, GTP-binding protein LepA (inferred from 100% identity to saz:Sama_0878)

MetaCyc: 80% identical to 30S ribosomal subunit biogenesis factor LepA (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Translation elongation factor LepA" in subsystem Heat shock dnaK gene cluster extended or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See A1S3X9 at UniProt or InterPro

Protein Sequence (596 amino acids)

>Sama_0878 GTP-binding protein LepA (RefSeq) (Shewanella amazonensis SB2B)
MKHIRNFSIIAHIDHGKSTLSDRLIQVCGGLTDREMAEQVLDSMDLERERGITIKAQSVT
LDYTAKNGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAL
EMDLEVVPVLNKIDLPQAEPERVAAEIEDIVGIEATDAVRCSAKTGVGIGEVLETIVAQI
PSPEGDPEAPLQALIIDSWFDSYLGVVSLVRIKNGVLKKGDKFKVMSTGQAYNADRVGIF
TPKMKDQAELKTGQVGYVIAGIKEIHGAPVGDTLTLAKHGADKPLPGFKKAKPQVYAGVF
TISTDDYESFRDALNKLSLNDASLQFEPETSSALGFGFRIGYLGLLHMEIIQERLEREYD
LDLITTAPTVEYEVLLTNGETLYVDNPSDLPATNYIEEMREPIVQANILVPKEYLGNVIT
LCIEKRGVQKNMVYHGNQVALTYDIPAAEVVMDFFDRLKSTSRGYASLEYNFIRFEPADM
VRLDVLINGDRVDALAMIIHRSNIRHKGIALVDKMKELIPRQMFDIAIQAAVGNQVVARS
TVKALRKDVTAKCYGGDVSRKKKLLQKQKEGKKRMKQLGNVEVPQEAFLAVLKLNE