Protein Info for Sama_0856 in Shewanella amazonensis SB2B

Name: gltD
Annotation: glutamate synthase subunit beta (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01318: glutamate synthase, small subunit
  • amino acids 5 to 468 (464 residues), 807.9 bits, E=1.2e-247
• PF14691: Fer4_20
  • amino acids 24 to 133 (110 residues), 132.8 bits, E=2.2e-42
• PF01494: FAD_binding_3
  • amino acids 147 to 178 (32 residues), 25 bits, see alignment (E = 4.8e-09)
• PF07992: Pyr_redox_2
  • amino acids 147 to 456 (310 residues), 96 bits, E=1.3e-30
• PF12831: FAD_oxidored
  • amino acids 148 to 216 (69 residues), 27.6 bits, E=8.4e-10
• PF13450: NAD_binding_8
  • amino acids 150 to 184 (35 residues), 29.8 bits, 2.7e-10
• PF00070: Pyr_redox
  • amino acids 287 to 359 (73 residues), 28.1 bits, E=1.1e-09

Best Hits

Swiss-Prot: 68% identical to GLTD_HALED: Glutamate synthase [NADPH] small chain (gltD) from Halomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9)

KEGG orthology group: K00266, glutamate synthase (NADPH/NADH) small chain [EC: 1.4.1.13 1.4.1.14] (inferred from 100% identity to saz:Sama_0856)

MetaCyc: 66% identical to glutamate synthase subunit GltD (Escherichia coli K-12 substr. MG1655)
Glutamate dehydrogenase (NADP(+)). [EC: 1.4.1.4]; Glutamate synthase (NADPH). [EC: 1.4.1.4, 1.4.1.13]

Predicted SEED Role

"Glutamate synthase [NADPH] small chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)

MetaCyc Pathways

• L-citrulline biosynthesis (7/8 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• superpathway of ammonia assimilation (plants) (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• ammonia assimilation cycle I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamate biosynthesis III (1/1 steps found)
• L-glutamate biosynthesis IV (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (1/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (1/4 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.1.14

Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14 or 1.4.1.4

Sequence Analysis Tools

See A1S3V7 at UniProt or InterPro

Protein Sequence (472 amino acids)

>Sama_0856 glutamate synthase subunit beta (RefSeq) (Shewanella amazonensis SB2B)
MSNDFQFIQVGRKDPTKQPIKLRATQFIEIYQPFTQPQVNEQADRCLDCGNPYCEWKCPL
HNYIPNWLKLAKQGRIMEAADLVHETNTLPEICGRVCPQDRLCEGACTLNDDFGAVTIGN
VEKYITDTALAQGWRPDMSKVTPRKERVAIVGAGPAGLGCADILARNGIKAVVFDKYPQI
GGLLTYGIPSFKLDKSVMQTRRTVLEGMGIEFRLGVTVGKDIAFADLLKDYDAVFLGMGT
YSAMKAGLPGEDADGVLQALPYLIGNTMSLLGQQSEQNPYIDLAGKKVVVLGGGDTAMDC
VRTAVRQGAREVTCAYRRDEANMPGSRREVQNAREEGVNFLFNRQPVAIKTENGAVVGIE
CIETRMGEPDANGRRSAEPVPGSEQLLEADAIIIAFGFQPSPAPWFADFDIALDDKGRVK
ASKADDNPFQTSNPKVFAGGDMVRGSDLVVTAIAEGREAALGILNYLESAKA