Protein Info for Sama_0795 in Shewanella amazonensis SB2B
Annotation: leucyl aminopeptidase (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 96% identical to AMPA2_SHEON: Probable cytosol aminopeptidase 2 (pepA2) from Shewanella oneidensis (strain MR-1)
KEGG orthology group: K01255, leucyl aminopeptidase [EC: 3.4.11.1] (inferred from 100% identity to saz:Sama_0795)MetaCyc: 77% identical to aminopeptidase A/I (Escherichia coli K-12 substr. MG1655)
Cytosol nonspecific dipeptidase. [EC: 3.4.13.18]; Leucyl aminopeptidase. [EC: 3.4.13.18, 3.4.11.1, 3.4.11.9]
Predicted SEED Role
"Cytosol aminopeptidase PepA (EC 3.4.11.1)" (EC 3.4.11.1)
MetaCyc Pathways
- γ-glutamyl cycle (5/6 steps found)
- glutathione degradation (DUG pathway) (2/2 steps found)
- muropeptide degradation (1/4 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.4.11.1, 3.4.11.9
Use Curated BLAST to search for 3.4.11.1 or 3.4.11.9 or 3.4.13.18
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A1S3P6 at UniProt or InterPro
Protein Sequence (502 amino acids)
>Sama_0795 leucyl aminopeptidase (RefSeq) (Shewanella amazonensis SB2B) MEFSVKSGSPEKQRSACIVVGVYEPRRLSGIAEQLDKISEGYISNLLRRGDLEGKPGQML LLHHVPNVLSERVLLVGCGKERELDERQYKQIIAKTINTLNETGSMEAVCFLTELHVKGR DTYWKVRQAVETTQNSLYSFDTLKSRKGETRRPLRKLVFNVPTRRELTVGERAIEHGVAV ASGMHLCRDVANMPPNICNPAYLASQARQLAEVHENLQVSTVGEEQMAKLGMNSYLAVGR GSANESIMTVMEYKGAVDSTAKPIVLVGKGLTFDSGGISLKPGEAMDEMKYDMGGAAGVI GTMKAICEMKLPLNVVGILAGCENMPSGNAYRPGDILTTMSGQTVEVLNTDAEGRLVLCD VLTYVERFDPELVVDTATLTGACVIALGKHASGLFSSHNPLAHELLSAGEQSGDRAWRMP LWDEYQDMLDSPFADMTNLGGRPAGSITAACFLSRFAKKYNWAHLDVAGTAWNSGANKGS TGRPVPLLTQFLINRAGVEQGE