Protein Info for Sama_0583 in Shewanella amazonensis SB2B

Annotation: diguanylate cyclase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 528 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 148 to 167 (20 residues), see Phobius details PF00672: HAMP" amino acids 166 to 219 (54 residues), 31.8 bits, see alignment 2.2e-11 TIGR00229: PAS domain S-box protein" amino acids 235 to 353 (119 residues), 29.8 bits, see alignment E=5.8e-11 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 357 to 518 (162 residues), 123.9 bits, see alignment E=5.3e-40 PF00990: GGDEF" amino acids 359 to 516 (158 residues), 132.5 bits, see alignment E=1.8e-42

Best Hits

KEGG orthology group: None (inferred from 100% identity to saz:Sama_0583)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S335 at UniProt or InterPro

Protein Sequence (528 amino acids)

>Sama_0583 diguanylate cyclase (RefSeq) (Shewanella amazonensis SB2B)
MIPNRQKLYVKVAVAIAVGAFLAAVVACLYFFWDARETKLREVKNQLGQIALTVQKTASV
ALYVKDVELGKEIVEGLEVNDLVAGALLTADSGDWVSSSGFAADDLLQVLEFDIPHPFFA
GEVLGKLKLLPNNEYIQSRATEVALKQAWMLVLQSALIALLVSLLVHKTLTAPLNRLTSN
FENIKPGDNDPLNVPKYHAQDEIGSLVRGINQLVANLNQSIEKERSLRQRTELLERKFRL
IFERATAGICLIDPGNHVLVANEAFQRLCGTESIDNQPALSHWFEEEAELARFFDELRGN
ANLNHVEMELQLRSQQSAQLKWVHCLFSKVEDRTQTLIEVMMYDITERTYRERMIRFEAE
HDPLTQLKNRRSGERLLVDMMRKVDQTRTLMGVLLIDLDKFKPVNDIYGHEAGDSVLKTV
AERLVKLVGDGIAIRWGGDEFLIGITDVDEDYNKLRLLAQEILVQVSRPIAIRGSLSCEV
DASVGIAIYPHHGHTLEALLEAADVAMYEVKEQGRGSFRIGRISSGAA