Protein Info for Sama_0560 in Shewanella amazonensis SB2B

Updated annotation (from data): D-mannose isomerase (EC 5.3.1.7)
Rationale: Specifically important for: D-Mannose. This is the first step in D-mannose catabolism. Also see PMID:20836887, PMCID: PMC4436071
Original annotation: hypothetical protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 389 PF07221: GlcNAc_2-epim" amino acids 30 to 380 (351 residues), 314.8 bits, see alignment E=4.1e-98

Best Hits

KEGG orthology group: None (inferred from 100% identity to saz:Sama_0560)

Predicted SEED Role

"D-mannose isomerase (EC 5.3.1.7)" in subsystem Mannose Metabolism (EC 5.3.1.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 5.3.1.7

Use Curated BLAST to search for 5.3.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S312 at UniProt or InterPro

Protein Sequence (389 amino acids)

>Sama_0560 D-mannose isomerase (EC 5.3.1.7) (Shewanella amazonensis SB2B)
MKFYNRDFLLSHSQSILDFYDPRVLDASGGYFHNYYDDGSLFEPGFRQLVSSCRITVNYA
RAADILDKPEYLAHARHGLNYLLNVHLQADERFAWTLKSHRPEDMTQQAYGYAFALLAFA
ACRKSGILKDNNKLLWIYNLLEQRFWQPEYGLYADEIGADGVLSDYRGQNANMHLCEAMI
AAFEASGEGRFLDRAMEIADKIANRQAALTGGIIWEHFTPGFAINWEYNKDDPKNLYRPW
GFQGGHQTEWAKLLLSLARHSHQPWLISRAKTLFDTAFEKSWDKEHGGMVYGFGPNGDWC
DDDKYFWVQAESFAAAAMLYQQTGEQKYLDAYNALWNYAWQHFVDHEHGAWFRVLYRDNR
KYSNEKSTAGAKCDYHTLGACFDSLRDLT