Protein Info for Sama_0428 in Shewanella amazonensis SB2B

Annotation: peptidase M4, thermolysin (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 775 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF07504: FTP" amino acids 51 to 93 (43 residues), 23.2 bits, see alignment 1.7e-08 PF03413: PepSY" amino acids 114 to 188 (75 residues), 29.3 bits, see alignment E=3e-10 PF01447: Peptidase_M4" amino acids 205 to 343 (139 residues), 128.4 bits, see alignment E=1.1e-40 PF02868: Peptidase_M4_C" amino acids 346 to 490 (145 residues), 122.2 bits, see alignment E=7.4e-39 PF04151: PPC" amino acids 524 to 590 (67 residues), 54.1 bits, see alignment E=8e-18 PF18911: PKD_4" amino acids 613 to 688 (76 residues), 68.6 bits, see alignment E=1.4e-22 PF00801: PKD" amino acids 625 to 689 (65 residues), 50.3 bits, see alignment E=7e-17

Best Hits

KEGG orthology group: K08604, vibriolysin [EC: 3.4.24.25] (inferred from 100% identity to saz:Sama_0428)

Predicted SEED Role

"Vibriolysin, extracellular zinc protease (EC 3.4.24.25) @ Pseudolysin, extracellular zinc protease (EC 3.4.24.26)" (EC 3.4.24.25, EC 3.4.24.26)

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.25, 3.4.24.26

Use Curated BLAST to search for 3.4.24.25 or 3.4.24.26

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S2N2 at UniProt or InterPro

Protein Sequence (775 amino acids)

>Sama_0428 peptidase M4, thermolysin (RefSeq) (Shewanella amazonensis SB2B)
MRKQQLSLLFIAVSATLGTSAYAANVVDVAKMRHATGDIGSVLQLAPGHEFRTAKQLDLG
KGLKKERLQQYFHGVPVYGFTVAADRSEMGFYSNLKGQMLANIDKDAAFAKPSLSKDKAL
EIAKAAKGGHGLKAGKTRNDSAQPWIILYGKAQEPRLVYITSYVVDGDTPSRPFTMVDAH
TGEVLERWEGLNHAGTGTGPGGNAKTGQYEYGTDFGNLDVEVNGDTCTMNNANVKTVNLN
HGTSGNTAFSYTCPRNTVKEINGAYSPLNDAHYFGGVVYDMYDQWYGTAPLSFQLTMRVH
YSNNYENAFWDGSAMTFGDGQSYFYPLVSLDVSAHEVSHGFTEQNSGLVYANQSGGMNEA
FSDMAGEAAEFFMKGSNDWLVGADIFKGDGALRYMADPTQDGKSIGHIDDYYDGLDVHYS
SGVFNKAFYTLATTEGWDTRKAFEVFVIANQLYWTPNSLMYEGACGVRNAATDKGYSTAD
VDAAFAAVGITPCEVPPPPPPPEAEVLQNGVAVTDISGASGSKQYWTLDVPAGASNLVFN
LSGGSGDADLYVKFGANPTSTDYDCRPYAAGNNENCAISNVQQGTYWVMLNGYSSYSGTT
LVGSFDGGSTEPNEAPVASFTADYTGALYNFTSTATDSDGNVVAWSWDFGDGETATGSAA
SHQYLVSGSYTVTLTVTDDDGATASTSEVFNVEVPAAEMDLSITKVNVSRRGSARIGLEW
TGNSASYTVLRNGEAVGTTSANSYVDRFTATAGSSVTFQVCNSDGGCSDIETVSF