Protein Info for Sama_0403 in Shewanella amazonensis SB2B

Annotation: drug resistance transporter, EmrB/QacA family protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 529 transmembrane" amino acids 21 to 41 (21 residues), see Phobius details amino acids 61 to 81 (21 residues), see Phobius details amino acids 90 to 109 (20 residues), see Phobius details amino acids 115 to 137 (23 residues), see Phobius details amino acids 149 to 169 (21 residues), see Phobius details amino acids 176 to 198 (23 residues), see Phobius details amino acids 210 to 230 (21 residues), see Phobius details amino acids 243 to 263 (21 residues), see Phobius details amino acids 284 to 304 (21 residues), see Phobius details amino acids 316 to 334 (19 residues), see Phobius details amino acids 345 to 362 (18 residues), see Phobius details amino acids 374 to 397 (24 residues), see Phobius details amino acids 410 to 430 (21 residues), see Phobius details amino acids 497 to 515 (19 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 26 to 512 (487 residues), 417.6 bits, see alignment E=3.6e-129 PF07690: MFS_1" amino acids 29 to 422 (394 residues), 145.8 bits, see alignment E=7.9e-47

Best Hits

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 100% identity to saz:Sama_0403)

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S2K7 at UniProt or InterPro

Protein Sequence (529 amino acids)

>Sama_0403 drug resistance transporter, EmrB/QacA family protein (RefSeq) (Shewanella amazonensis SB2B)
MSASIALPQTDVDYGVGSRRAWIAVMGGLIGAFMAILDIQITNASMKEIQGSLGATLEEG
SWISTAYLVAEMIAIPLSGWLSTGLSVRRYLLWSTTAFILSSLLCSMAWDLNSLIAFRAL
QGFFGGALIPLAFRLIVECLPFEKRAMGMALFGVTATFAPSIGPTLGGWLTEHFSWHFLF
YINVPPGLLVLWMLAYGLQKGPIDWGKLGKVSWVGIATMALGLGCLEVVLEEGNRKDWLG
SDVIRDLAIIAAINLVLFVYLQLRSRNPLVNLRLLAHRDFALSSLSYFLLGLGLFGAIYL
IPLYLSQIHNYSALEIGKVIMWMGFPQLLVLPLVPKLTQKLDPRYLAAWGFLLFALSYYL
NAHMTAQYAGEQMILAQIVRALGQPFVLVPIGMIATMHLRQEDTPSASTLLNLIRNLGGA
CGIALVATMLDNRFRQHLAYLKESLPAVSQQAHTFLQQQASSLFAAGADPMAADSMARKM
LSDTLVREAYMQAYNDVFFAIGGLLLVAVVAVLLIRPSSQAGNSGAAGH