Protein Info for Sama_0264 in Shewanella amazonensis SB2B

Annotation: bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 699 TIGR00691: RelA/SpoT family protein" amino acids 26 to 695 (670 residues), 882.7 bits, see alignment E=8.7e-270 PF13328: HD_4" amino acids 26 to 175 (150 residues), 181.5 bits, see alignment E=2.9e-57 PF01966: HD" amino acids 45 to 144 (100 residues), 47.2 bits, see alignment E=7.9e-16 PF04607: RelA_SpoT" amino acids 235 to 345 (111 residues), 141.4 bits, see alignment E=4.3e-45 PF02824: TGS" amino acids 389 to 448 (60 residues), 72.3 bits, see alignment 8.1e-24 PF19296: RelA_AH_RIS" amino acids 460 to 539 (80 residues), 39.4 bits, see alignment E=2.1e-13 PF13291: ACT_4" amino acids 621 to 697 (77 residues), 61.8 bits, see alignment E=2.4e-20

Best Hits

Swiss-Prot: 70% identical to SPOT_ECOLI: Bifunctional (p)ppGpp synthase/hydrolase SpoT (spoT) from Escherichia coli (strain K12)

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 100% identity to saz:Sama_0264)

MetaCyc: 70% identical to bifunctional (p)ppGpp synthase/hydrolase SpoT (Escherichia coli K-12 substr. MG1655)
Guanosine-3',5'-bis(diphosphate) 3'-diphosphatase. [EC: 3.1.7.2]; 3.1.7.2 [EC: 3.1.7.2]; GTP diphosphokinase. [EC: 3.1.7.2, 2.7.6.5]

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S269 at UniProt or InterPro

Protein Sequence (699 amino acids)

>Sama_0264 bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (RefSeq) (Shewanella amazonensis SB2B)
MYLFEGLKESASAYLDSGQVALLKQAYQVARDAHEGQMRTSGEPYITHPVAVAKILSDMR
LDHETLMAALLHDTIEDTHVTKEDLAELFGDAVADLVEGVSKLDKLKFRDKKEAQAENFR
KMMMAMTQDIRVILIKLADRTHNMRTLGALRPDKRRRIARETLEIYAPIANRLGIHNIKT
ELEDLGFQAYYPMRYRVLKEVVKAARGNRKELIQNIKTGIQARLEDAGIHAKVKGREKNL
YSIYNKMRGKELQFQEVMDIYAFRVIVDSIDTCYRVLGAMHGLYKPRPGRFKDYIAIPKA
NGYQSLHTSLFGPHGVPVEIQIRTDDMDQMADKGVAAHWVYKNGNENGSTTTQVRARKWM
QSLLELQQSASSSFEFVENVKTELFPDEIYVFTPEGRILELPVGATAVDFAYEVHTDVGN
TCVGARVNRQAYPLSQPLISGQTVEIITAKGAKPNAAWLNFVVTGKARAKIRQVLKNLKQ
DEAIALGRRLLNHALGENKLDSVAPELLDKVIQETKHKDLNSLLADIGLGNAMSIVIAQR
LTGGAVPEKQSETHLMPIRGAEGMLVSFAKCCHPIPGDAVIAHVSPGKGLVVHMENCANI
RGYQGEPDKYIPVHWDKVDGVEYQANLRVEIVNHQGALAKITSIIASEGSNIHNLSTEER
DGRVYLINLRISVRDRIHLANVMRRIRVLPEVLRTSRNR