Protein Info for Sama_0165 in Shewanella amazonensis SB2B

Updated annotation (from data): Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49)
Rationale: Specifically important for utilizing Tween 20. Automated validation from mutant phenotype: the predicted function (PEPCARBOXYKIN-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: phosphoenolpyruvate carboxykinase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to PCKA_SHEAM: Phosphoenolpyruvate carboxykinase (ATP) (pckA) from Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)

KEGG orthology group: K01610, phosphoenolpyruvate carboxykinase (ATP) [EC: 4.1.1.49] (inferred from 100% identity to saz:Sama_0165)

Predicted SEED Role

"Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP or Serine-glyoxylate cycle (EC 4.1.1.49)

MetaCyc Pathways

gluconeogenesis I (13/13 steps found)
superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
C4 photosynthetic carbon assimilation cycle, PEPCK type (9/14 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1S1X0 at UniProt or InterPro

Protein Sequence (513 amino acids)

>Sama_0165 Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) (Shewanella amazonensis SB2B)
MAEGINRVHRNPSTAELVELALRRGEGELTANGALVAKTGARTGRSPNDRFIVKEAGSEA
DIEWGNVNKPFAPEAFNALWDRVAAYLADKEVFVSDLEVGADPEHYLPVTVTTEYAWHQL
FARNLFIIPEHFNQAGKPTWQIMNAPGFVCEPERDGTASEATVIINFAERKVLLAGLKYA
GEMKKSMFSVQNFLLPAKGVLPMHCSANVGKDGDTTLFFGLSGTGKTTLSADPKRFLIGD
DEHGWAPGSVFNIEGGCYAKCIDLSQKNEPVIWDAIRFGTVLENVVLDDKRVPDYKNASL
TENTRAAYPLEHIAQRQEENRGGEPRAVVFLTCDVSGVLPPVSKLTKEQAAYHFLSGYTA
KVGSTEMGSTAAIQSTFSTCFGAPFFPRPAGVYAELLMKRIEEFGSQVYLVNTGWTGGPY
GVGKRFDIPTTRAIVDAIVSGELASVETVHLEKLNLEVPVAVPGVETALLNPVNTWADKA
KYQEYAQKLAEEFQANFAKYQVPDSIKNAGPKA