Protein Info for Shew_3842 in Shewanella loihica PV-4

Annotation: UDP-N-acetylglucosamine pyrophosphorylase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 4 to 451 (448 residues), 635.8 bits, E=2e-195
• PF01128: IspD
  • amino acids 4 to 133 (130 residues), 27.5 bits, E=6.3e-10
• PF00483: NTP_transferase
  • amino acids 5 to 168 (164 residues), 52.2 bits, E=1.6e-17
• PF12804: NTP_transf_3
  • amino acids 5 to 127 (123 residues), 71.3 bits, E=2.8e-23
• PF00132: Hexapep
  • amino acids 263 to 295 (33 residues), 29.5 bits, see alignment (E = 1.1e-10)
  • amino acids 392 to 426 (35 residues), 34.1 bits, 3.7e-12
• PF14602: Hexapep_2
  • amino acids 393 to 426 (34 residues), 28 bits, 3.6e-10

Best Hits

Swiss-Prot: 100% identical to GLMU_SHELP: Bifunctional protein GlmU (glmU) from Shewanella loihica (strain ATCC BAA-1088 / PV-4)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 100% identity to slo:Shew_3842)

MetaCyc: 62% identical to fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylglucosamine diphosphorylase. [EC: 2.7.7.23]; Glucosamine-1-phosphate N-acetyltransferase. [EC: 2.7.7.23, 2.3.1.157]

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• peptidoglycan recycling I (10/14 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (5/9 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (6/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Sequence Analysis Tools

See A3QJQ7 at UniProt or InterPro

Protein Sequence (454 amino acids)

>Shew_3842 UDP-N-acetylglucosamine pyrophosphorylase (RefSeq) (Shewanella loihica PV-4)
MALNVVILAAGKGTRMRSDLPKVLHPIAHKSMVQHVIDTAHQLGSEAIQLVYGYGADKLQ
ARLGEQALNWVLQAEQLGTGHAVAQANDNIGDDDTVLILYGDVPLIQVSTLEALLAARDA
NGLAILTVNLHDPTGYGRIVREAGKVVGIVEQKDANEEQLKINEINTGIMAAPGKQLKAW
LGQLSSDNAQGEYYLTDIVAMAHSDGVSITTAQPESAIEVEGANNRVQLAQLERAYQARA
AEKLMLEGANLRDPARIDVRGEVTVGMDVMIDVNVVFQGKVTIGNNVTIGAGAILIDCEI
GDNAEIKPYSIIENAIVGEAASAGPFARLRPGAELKRDAHIGNFVEMKKAVLGEGSKAGH
LAYIGDAQVGAGVNIGAGTITCNYDGANKHLTVIEDDVFVGSDTQLVAPVTIGKGATLGA
GSTITRDVAADELVITRVKQRHLTGWTRPVKQKK