Protein Info for Shew_3757 in Shewanella loihica PV-4

Annotation: urocanate hydratase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 556 TIGR01228: urocanate hydratase" amino acids 10 to 553 (544 residues), 971.5 bits, see alignment E=6e-297 PF17391: Urocanase_N" amino acids 12 to 137 (126 residues), 205.5 bits, see alignment E=3e-65 PF01175: Urocanase" amino acids 140 to 348 (209 residues), 300.1 bits, see alignment E=1.3e-93 PF17392: Urocanase_C" amino acids 351 to 545 (195 residues), 306.4 bits, see alignment E=1.1e-95

Best Hits

Swiss-Prot: 96% identical to HUTU_SHEWM: Urocanate hydratase (hutU) from Shewanella woodyi (strain ATCC 51908 / MS32)

KEGG orthology group: K01712, urocanate hydratase [EC: 4.2.1.49] (inferred from 100% identity to slo:Shew_3757)

MetaCyc: 77% identical to urocanase subunit (Pseudomonas putida)
Urocanate hydratase. [EC: 4.2.1.49]

Predicted SEED Role

"Urocanate hydratase (EC 4.2.1.49)" in subsystem Histidine Degradation (EC 4.2.1.49)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QJH5 at UniProt or InterPro

Protein Sequence (556 amino acids)

>Shew_3757 urocanate hydratase (RefSeq) (Shewanella loihica PV-4)
MDKRHDPSRRIIAPHGTTLSCKSWLTEAPMRMLMNNLHPDVAERPEDLVVYGGIGRAARD
WQCYDKIVEVLQRLEEDETLLVQSGKPVGVFKTHSNAPRVIIANSNLVPHWANWEHFNEL
DKKGLAMYGQMTAGSWIYIGSQGIVQGTYETFVAMAKQHFGGDASGKWILTGGLGGMGGA
QPLAGTMAGYSVLACEVDETRIDFRLRTRYVDKKATSLDEALAMIDEANKSGKPVSVGLL
ANAADIFAELVERGITPDVVTDQTSAHDPLNGYLPQGWTLEYAAEMRKQDEAAVVKAAKQ
SMAVQVKAMLALQAAGAATTDYGNNIRQMAFEEGVENAFDFPGFVPAYVRPLFCEGIGPF
RWAALSGDPEDIYKTDAKVKELIPDNPHLHNWLDMARERIAFQGLPARICWVGLKDRARL
AKAFNEMVKNGELSAPIVIGRDHLDSGSVASPNRETESMLDGSDAVSDWPLMNALLNTAS
GATWVSLHHGGGVGMGFSQHSGVVIVADGTDEAEARLGRVLWNDPATGVMRHADAGYEIA
KQCAKEQGLDLPMLDL