Protein Info for Shew_3730 in Shewanella loihica PV-4

Annotation: Sua5/YciO/YrdC/YwlC family protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 185 TIGR00057: tRNA threonylcarbamoyl adenosine modification protein, Sua5/YciO/YrdC/YwlC family" amino acids 8 to 181 (174 residues), 138.3 bits, see alignment E=9.5e-45 PF01300: Sua5_yciO_yrdC" amino acids 13 to 185 (173 residues), 178.1 bits, see alignment E=6e-57

Best Hits

Swiss-Prot: 100% identical to TSAC_SHELP: Threonylcarbamoyl-AMP synthase (tsaC) from Shewanella loihica (strain ATCC BAA-1088 / PV-4)

KEGG orthology group: K07566, putative translation factor (inferred from 100% identity to slo:Shew_3730)

MetaCyc: 52% identical to L-threonylcarbamoyladenylate synthase (Escherichia coli K-12 substr. MG1655)
RXN-14569 [EC: 2.7.7.87]

Predicted SEED Role

"TsaC protein (YrdC domain) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.87

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QJE8 at UniProt or InterPro

Protein Sequence (185 amino acids)

>Shew_3730 Sua5/YciO/YrdC/YwlC family protein (RefSeq) (Shewanella loihica PV-4)
MLQQDPAALVETVLQGGVIAYPTEAVYGLGCDPDNDEAIQKLLDLKQRPWQKGLILVASD
FNQLTPYIDSSVLTEAQLQSAFDKWPGPFTFIMPKRPGLSSLLSGQFDSLAVRVSNHPGV
QALCQALGKPIVSTSANLTGEEPALTPEEVIRQFDGKIDGLVAGSLGAEPKPSTIIDVIS
GKILR