Protein Info for Shew_3588 in Shewanella loihica PV-4

Annotation: Bcr/CflA subfamily drug resistance transporter (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details transmembrane" amino acids 41 to 61 (21 residues), see Phobius details amino acids 71 to 91 (21 residues), see Phobius details amino acids 100 to 117 (18 residues), see Phobius details amino acids 129 to 153 (25 residues), see Phobius details amino acids 159 to 178 (20 residues), see Phobius details amino acids 208 to 228 (21 residues), see Phobius details amino acids 246 to 266 (21 residues), see Phobius details amino acids 274 to 295 (22 residues), see Phobius details amino acids 301 to 325 (25 residues), see Phobius details amino acids 339 to 359 (21 residues), see Phobius details amino acids 365 to 385 (21 residues), see Phobius details TIGR00710: drug resistance transporter, Bcr/CflA subfamily" amino acids 7 to 383 (377 residues), 246.8 bits, see alignment E=2.5e-77 PF07690: MFS_1" amino acids 9 to 354 (346 residues), 160.4 bits, see alignment E=9.1e-51 PF00083: Sugar_tr" amino acids 38 to 182 (145 residues), 26.6 bits, see alignment E=4.2e-10 PF06609: TRI12" amino acids 44 to 180 (137 residues), 26.8 bits, see alignment E=2.7e-10

Best Hits

KEGG orthology group: K07552, MFS transporter, DHA1 family, bicyclomycin/chloramphenicol resistance protein (inferred from 100% identity to slo:Shew_3588)

Predicted SEED Role

"Putative multidrug resistance protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QJ06 at UniProt or InterPro

Protein Sequence (400 amino acids)

>Shew_3588 Bcr/CflA subfamily drug resistance transporter (RefSeq) (Shewanella loihica PV-4)
MRRNLLPILMLMVVLSPLAIDIYLPSMPVMAAEFAVSASQVQSTLVLFLFAMGVGQVLIG
PLADRYGRRPIALGGLLLYIASSLLAAVAMEFQWLQLARVLQGLAACSTSIVVFSAVRDC
FTPKEGARYYSYLNGVICVIPALAPTLGGLLALQFGWRSTFVFMTLYAIIIMIIVGYRLP
ETRPANTVTEGPLYRWSRYKPVLMDPHFMFYAFACMAGMAAILAYVSYSPVWLIETIGVS
ELTFSGLFGLNALVNIGACFAAPVVIQKLGNRPTVIVALSAMLVSALLQLAVQWAGPQTG
LAAAFGFMLPMMLLCIGFALLLGPATSMALAGFGERAGTATAMLGFIQMSGASILAGLVQ
QTSLTAPYAVVVVMGGLASLLLVAMNMSRLDTWHQERHAH