Protein Info for Shew_3534 in Shewanella loihica PV-4

Annotation: CzcA family heavy metal efflux protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1047 transmembrane" amino acids 12 to 28 (17 residues), see Phobius details amino acids 339 to 359 (21 residues), see Phobius details amino acids 365 to 383 (19 residues), see Phobius details amino acids 391 to 412 (22 residues), see Phobius details amino acids 446 to 466 (21 residues), see Phobius details amino acids 484 to 505 (22 residues), see Phobius details amino acids 535 to 554 (20 residues), see Phobius details amino acids 875 to 892 (18 residues), see Phobius details amino acids 899 to 918 (20 residues), see Phobius details amino acids 924 to 948 (25 residues), see Phobius details amino acids 983 to 1001 (19 residues), see Phobius details amino acids 1013 to 1036 (24 residues), see Phobius details TIGR00914: heavy metal efflux pump, CzcA family" amino acids 1 to 1045 (1045 residues), 1230.2 bits, see alignment E=0 PF00873: ACR_tran" amino acids 5 to 1036 (1032 residues), 781.8 bits, see alignment E=1e-238

Best Hits

Swiss-Prot: 57% identical to CUSA_ECOLI: Cation efflux system protein CusA (cusA) from Escherichia coli (strain K12)

KEGG orthology group: K07787, Cu(I)/Ag(I) efflux system membrane protein CusA (inferred from 58% identity to adn:Alide_2726)

MetaCyc: 57% identical to copper/silver export system RND permease (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-90; TRANS-RXN0-280

Predicted SEED Role

"Cobalt-zinc-cadmium resistance protein CzcA; Cation efflux system protein CusA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QIV2 at UniProt or InterPro

Protein Sequence (1047 amino acids)

>Shew_3534 CzcA family heavy metal efflux protein (RefSeq) (Shewanella loihica PV-4)
MLKKIIEASIAQRLMVLIIALMITVWGVQELRKTPLDALPDLSDVQVIIKTPYPGQAPKL
VEEQVTYPLSTAMLAVPGAKTVRGYSFFGDSYVYVIFEDGTDIYWARSRVLEYLSQVSSR
LPQGLQPSLGPDASGVGWIYEYALVDRSGNLDLSQLKSLQDWYLKLELQSVAGVSEVATV
GGMEQTYQIVIEPDKLAIYKLDIAAVKNAINKSNTEAGGSVIEMAEAEYMVRAKGYRQTL
DDFREIPLGVTSPSGTALLLKDVATVRKGPASRRGIAELDGEGEVVGGIIVMRYGENALA
TIDAVKAKLEQLKAGLPQGVEIIPTYDRSELINKSVDNLFHKVIEEMLVVGLVCLLFLLH
ARSTLVAVITLPLSILIAFIVMNKMGVNANIMSLGGIAIAIGAVVDGAIVMIENMHKHLE
HFKDEYRRDPDNKEHWHIVAKASIEVGPALFFSLLIITLSFVPVFALEAQEGRLFSPLAY
TKTFAMAAAAILSITLVPILMGYFIRGKIPKETSNPISRVLIALYKPALNMVLRFPKITL
MLAVIALGSAWYPVTKMGSEFMPELEEGDLLYMPTALPGISASKAAEVLQQTDRLIKTVP
EVKRVFGKVGRAETATDPAPLTMLETTIMLKPREEWREGVDLQGVIDQLQKTVKVPGLTN
AWVQPIKTRIDMLSTGIKTPVGIKITGADVNELQKVGADIEAILSQLPNTKSAYAERVGG
GRYVDITPKLDVASRYGMTLTDIQDVVRYAIGGMDIGESIQGAERYPINLRYPRALRDNI
EKLRELPVITKSGHYLPLRNLADIEITDGAPMLKSENGRLISWVFVDIQNTSIGEYIDEA
KSALDQQLKVPPRYSYSFAGQYEYMQRVDAKLKKVIPMALGVIFILLMMTFGSPLQASII
MLSLPFALVGSTWLLFALDFNMSVAVAVGMIALAGVAAEFGVVMLVYLNNAIKHRKDTGG
YEYVADLKEALIEGAVMRIRPKAMTVATIFFGLLPIMWGAGSGNDVMQKIAAPMVGGMIT
APLLSLFVLPALYLLIYGRRLKHSAEA