Protein Info for Shew_3458 in Shewanella loihica PV-4

Annotation: UDP-N-acetylmuramyl-tripeptide synthetase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 488 transmembrane" amino acids 298 to 320 (23 residues), see Phobius details TIGR01085: UDP-N-acetylmuramyl-tripeptide synthetase" amino acids 19 to 483 (465 residues), 502.3 bits, see alignment E=7.5e-155 PF01225: Mur_ligase" amino acids 20 to 96 (77 residues), 45.3 bits, see alignment E=1.4e-15 PF08245: Mur_ligase_M" amino acids 110 to 311 (202 residues), 185.1 bits, see alignment E=2.3e-58 PF02875: Mur_ligase_C" amino acids 332 to 418 (87 residues), 91.4 bits, see alignment E=5.6e-30

Best Hits

Swiss-Prot: 65% identical to MURE_SHEON: UDP-N-acetylmuramyl-tripeptide synthetase (murE) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01928, UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase [EC: 6.3.2.13] (inferred from 100% identity to slo:Shew_3458)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase (EC 6.3.2.13)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QIM6 at UniProt or InterPro

Protein Sequence (488 amino acids)

>Shew_3458 UDP-N-acetylmuramyl-tripeptide synthetase (RefSeq) (Shewanella loihica PV-4)
MHLCDLLAPWFHYAGGESLSHLTLDSREIGQGSVFVAVPGHRVDGRDYVDTALSQGAVAA
LIHTDDPAEHGKVSQDNGLRIYFFQLQRQLSALASQLYVLGRDRIKLIGVTGTNGKTSVT
QLIAQLSDGLGKPAAVMGTLGNGLWGQLVDSGNTTADPITVMRQLEDFDRQGTAVCAMEV
SSHGLVQGRVDAVPFDVAVFTNLSRDHLDYHGSMDAYGAAKQRLFRFSSLSHGLINLDDP
VGRQWIQDKRCKGLVGFSIQGQEGAAIYTKEVKQHSQGVSATLVWPEGEAKIDSPLLGIF
NLSNLVAALGALYLSGYAMAQLLTQVSSLKPVAGRMERFTTQEGVTLVVDYAHTPDAIEQ
ALRALRGHCRGKLWCVFGCGGDRDKGKRPLMARAAEENADCVMVTSDNARSEDPMAIIQD
VLAGLNKPQIALTQVDRQQAIREVVTLASNGDLILLAGKGHETYQEIAGKRLDYDERALA
AAIARGGK