Protein Info for Shew_3438 in Shewanella loihica PV-4

Annotation: type IV-A pilus assembly ATPase PilB (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 569 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR02538: type IV-A pilus assembly ATPase PilB" amino acids 10 to 568 (559 residues), 871.3 bits, see alignment E=1.6e-266 PF05157: MshEN" amino acids 62 to 146 (85 residues), 54.8 bits, see alignment E=7.9e-19 PF00437: T2SSE" amino acids 180 to 565 (386 residues), 524.2 bits, see alignment E=1.5e-161

Best Hits

Swiss-Prot: 67% identical to TAPB_AERHY: Type IV pilus assembly protein TapB (tapB) from Aeromonas hydrophila

KEGG orthology group: K02652, type IV pilus assembly protein PilB (inferred from 100% identity to slo:Shew_3438)

Predicted SEED Role

"Type IV fimbrial assembly, ATPase PilB" in subsystem Type IV pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QIK6 at UniProt or InterPro

Protein Sequence (569 amino acids)

>Shew_3438 type IV-A pilus assembly ATPase PilB (RefSeq) (Shewanella loihica PV-4)
MPSTGLHLGLSTLFIRKGLLSEEQLSEAVDLSRKNKQPLVSTLIATKVLSAREVAELCYE
EYGTPLLDLNEFDISSIPEAFLNKKLIEKHKCLPLFKRGNRLYIGTSDPTNIAALEDFQF
SAGLHAEAILVEDDKLTVALEKVLEEDISALDLDGIDEDSLSGIEITDTDKRQEEQQGDA
SDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSGRISA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGI
EKLGYENDQRKLYEEMLAKPQGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVE
INLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRDLETAEIAIKAAQTGHLVLS
TLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCTECRQPEEVPEHELLKLGF
TQAQIDEGFTVYKPVGCDLCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIATQAKEQ
GMRDLRESGLRKVIAGVTSIAEINRVTSF