Protein Info for Shew_3434 in Shewanella loihica PV-4

Annotation: N-acetyl-anhydromuranmyl-L-alanine amidase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01510: Amidase_2
  • amino acids 29 to 170 (142 residues), 108.6 bits, E=1.5e-35

Best Hits

Swiss-Prot: 60% identical to AMPD_ECOLI: 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD (ampD) from Escherichia coli (strain K12)

KEGG orthology group: K03806, AmpD protein (inferred from 100% identity to slo:Shew_3434)

MetaCyc: 60% identical to 1,6-anhydro-N-acetylmuramoyl-L-alanine amidase (Escherichia coli K-12 substr. MG1655)
N-acetylmuramoyl-L-alanine amidase. [EC: 3.5.1.28]

Predicted SEED Role

"N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) AmpD" (EC 3.5.1.28)

MetaCyc Pathways

• peptidoglycan recycling I (10/14 steps found)
• peptidoglycan recycling II (4/10 steps found)

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.28

Use Curated BLAST to search for 3.5.1.28

Sequence Analysis Tools

See A3QIK2 at UniProt or InterPro

Protein Sequence (184 amino acids)

>Shew_3434 N-acetyl-anhydromuranmyl-L-alanine amidase (RefSeq) (Shewanella loihica PV-4)
MANMTLHKGWIRECRQCVSPHFNARPDDEVSLLVIHNISLPAGQFGLPYIDQLFQGCLDI
QADESFCDLQGLEVSAHFLIRRDGELVQYVSCDDRAWHAGVSSFAGRTGCNDFAIGIELE
GTDSLAYTDEQYERLTQLTLVLFDAYPMLNRNLIVGHSDIAPGRKTDPGESFDWHRYRVS
LVTG