Protein Info for Shew_3405 in Shewanella loihica PV-4

Annotation: EmrB/QacA family drug resistance transporter (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 538 transmembrane" amino acids 36 to 56 (21 residues), see Phobius details amino acids 76 to 96 (21 residues), see Phobius details amino acids 105 to 124 (20 residues), see Phobius details amino acids 130 to 152 (23 residues), see Phobius details amino acids 164 to 185 (22 residues), see Phobius details amino acids 191 to 213 (23 residues), see Phobius details amino acids 225 to 245 (21 residues), see Phobius details amino acids 258 to 278 (21 residues), see Phobius details amino acids 299 to 319 (21 residues), see Phobius details amino acids 334 to 353 (20 residues), see Phobius details amino acids 360 to 376 (17 residues), see Phobius details amino acids 396 to 416 (21 residues), see Phobius details amino acids 428 to 445 (18 residues), see Phobius details amino acids 511 to 530 (20 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 40 to 526 (487 residues), 410.4 bits, see alignment E=5.4e-127 PF07690: MFS_1" amino acids 44 to 437 (394 residues), 151.2 bits, see alignment E=3.7e-48 PF00083: Sugar_tr" amino acids 52 to 208 (157 residues), 30.6 bits, see alignment E=1.7e-11

Best Hits

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 100% identity to slo:Shew_3405)

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QIH3 at UniProt or InterPro

Protein Sequence (538 amino acids)

>Shew_3405 EmrB/QacA family drug resistance transporter (RefSeq) (Shewanella loihica PV-4)
MSSVSATQEIPAEIAEQVVPPQATKPQGYERGSFRSWVAVFAGLLGAFMAILDIQITNAS
MKEIQGSLGATLEEGSWIATAYLVAEMIAIPLSGWLSQGLSVRRYLLWTTAAFILASFLC
SLAWNLEAMIAFRALQGFFGGALIPLAFRLILELLPKEKRATGMALFGVTATFAPSIGPT
LGGWLTEQFSWHYLFYINIPPGLLVMAMLAYGLRAQAIDWDKLKNADYSGIVTMALGMGC
LEVVLEEGNRKDWFGSELIQNLAIVAAINIALFVFIQLRKSSPLVNLRLLGQRDFALSTL
AYFLLGMALFSAIYMIPLYLSQIHDYSPLEIGEVIMWMGFPQLLVLPLVPRLLQRFDGRY
LAAFGFSMFALSYYMNSHMTLDFAGEQMIAAQVVRALGQPFILVPIGIIATMHISAQQTP
SASTVLNVIRNLGGAFGIALVATLIDNQSRTHLADMKQTVAAVSGQAYQYLQQTSQLFVQ
AGSDPVTAQAQAKAMLAKTMTQQAYIQAYNDVFLIISVLLMLAVVAVLMIRKPKPDAA