Protein Info for Shew_3400 in Shewanella loihica PV-4

Annotation: nitrous-oxide reductase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 628 signal peptide" amino acids 1 to 50 (50 residues), see Phobius details TIGR04244: nitrous-oxide reductase, TAT-dependent" amino acids 16 to 627 (612 residues), 1069.6 bits, see alignment E=0 PF18793: nos_propeller_2" amino acids 167 to 237 (71 residues), 111.8 bits, see alignment E=1.7e-36 PF18764: nos_propeller" amino acids 415 to 485 (71 residues), 120.7 bits, see alignment E=1.9e-39

Best Hits

Swiss-Prot: 67% identical to NOSZ_PSEFL: Nitrous-oxide reductase (nosZ) from Pseudomonas fluorescens

KEGG orthology group: K00376, nitrous-oxide reductase [EC: 1.7.2.4] (inferred from 100% identity to slo:Shew_3400)

MetaCyc: 67% identical to nitrous oxide reductase subunit (Stutzerimonas stutzeri)
RXN-12130 [EC: 1.7.2.4]

Predicted SEED Role

"Nitrous-oxide reductase (EC 1.7.99.6)" in subsystem Denitrification (EC 1.7.99.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.2.4 or 1.7.99.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QIG8 at UniProt or InterPro

Protein Sequence (628 amino acids)

>Shew_3400 nitrous-oxide reductase (RefSeq) (Shewanella loihica PV-4)
MQDKNNKPANQLENAGRREFMGKTALLGAGAVAAPMSAAMFASMAKAQAAEASNSPVVHP
GELDEYYGFWSGGHSGEVRIMGLPSMRELMRIPVFNIDSATGWGITNESKAVKGESAHLM
AGDAHHPHMSMQDGRYDGKYLFINDKANTRVARIRCDVMKTDKMITIPNAQAIHGLRVQK
VPYTKYVICNGEFEVPLNNNGKETLEDVSTYRSLFNVIDAEKMEVAFQVMVDGNLDNTDA
DYDGKYFASTCYNSEMGMNLSEMITAERDHVVIFNLARCEAAVKAGKFKTYNGNKIPVLD
GRKGSDLTRYIPVPKSPHGLNTSPSGKYFVANGKLSPTVSIISIEKLDDLFDDKIKPRDA
IVGEPELGLGPLHTAFDNKGYAYTTLFLDSQIAKWNVEEAIQAYQGKKVNYLRQKLDVHY
QPGHNHTSQGETRDADGKWLVSLCKFSKDRFLPVGPLRPENDQLIDISGDEMKLVHDGPT
FAEPHDCIIVHRSKLKPKKLWTRDDPLFADTVAMAEKDGVRLMMDNKVIRDGNKVRVYMT
SVAPNYGMSEFKVKLGDEVTVVVTNLDQVEDVTHGFCMTNHGVQMEIGPQATASVTFIAD
KPGVQWYYCNWFCHALHMEMRGRMLVEA