Protein Info for Shew_3269 in Shewanella loihica PV-4

Annotation: Beta-galactosidase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1076 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details PF02837: Glyco_hydro_2_N" amino acids 74 to 241 (168 residues), 146.8 bits, see alignment E=1.5e-46 PF00703: Glyco_hydro_2" amino acids 244 to 352 (109 residues), 67.9 bits, see alignment E=3.2e-22 PF02836: Glyco_hydro_2_C" amino acids 354 to 637 (284 residues), 317.6 bits, see alignment E=2e-98 PF16353: LacZ_4" amino acids 651 to 740 (90 residues), 54.3 bits, see alignment E=3.4e-18 PF02929: Bgal_small_N" amino acids 769 to 1061 (293 residues), 196.4 bits, see alignment E=1.8e-61

Best Hits

KEGG orthology group: K01190, beta-galactosidase [EC: 3.2.1.23] (inferred from 100% identity to slo:Shew_3269)

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QI37 at UniProt or InterPro

Protein Sequence (1076 amino acids)

>Shew_3269 Beta-galactosidase (RefSeq) (Shewanella loihica PV-4)
MINRFSLLQRLSYTLAFSLVFGLVVSNPALARDRWQDHTLFEVNKLAPHASFFGYESEPL
ALLDEMDRSQLYLDLNGRWRFHLAKNPDATPKEFAAPEFDASHWGSIQVPGNFETQGYGH
AIYLDERYPFDTKWPDAPSDHNPTGLYRKTFTLPAHWQQKQVFIHIGAARSALTLFVNGR
EVGYSQGAKTPAEFDITPYLQAGDNLVAMQLIRWSDASYLESQDMLRMTGIEREVYLYAT
PKQRIEDIQVVTHLNEDLTRAKLAIRVDIASHQPGVRALELEARLLDPQGKPVAKANQRL
SLKGDAKPVFSQTLISPKLWNAEMPNLYRLILTLKTEKGETLQVASQQIGVRKIAIENGQ
LKVNNKAITIRGVDRHETDPQTGHVVSRETMELDIRLMKQNNINAVRSSHYPNHPYWLSL
ADRYGLYVIDEANIESHPLAIDDKTQLGNEMSWLPAHQARIERMVERDKNHPSVIIWSLG
NEAGEGKLFERLYQWIKRRDPNRPVQYEPAGEAPYTDIVAPMYPSIERIREYAERASDRP
LIMIEYAHAMGNSVGNLQDYWDVIEAYPQLQGGFIWDWVDQALAFSNDLGQRYWAYGKDY
HPDMPTDGNFLNNGLVDPDRNPHPHLSEVKKVYQPIKLRDFKVEGDKASVNLINGFDFAS
TSGLSLNWTLQKDGKIIASKRQAMPILSPGEQARVSLTLPGKQSLSAPFEYHLLLEVLVD
TPRPLLPSDLRIAFEQFALPRFGTKAAYQVKKNQAKISEDSGSYRLTSGELSYEFDKRSG
WLTQIYQEGEPQLKAPLMANFWRAPTDNDLGNQMPDWAGAWQDAATELEVTAIDADLALG
LTISQTHAEKGFSLRTRYSLDNAGRLMVDSQFIPGNKPLADLPRFGFSTRLGFEHRYLSY
FGRGPEETYADRQSGNPLGWYALPIEQTYHRYPRPQETGQRTQVRYAAVTDQRGQGWLAI
ANQAHAGEQDRKEADEVATLQTSLWPFAQADIDFRRGDAQDSASGLVAVTRNHGAEIPLR
EFVTWNIDYRQMGVGGDTSWGRPVHGPYRIKAEPIRFGFTLMPVSSDDDIRTLARQ