Protein Info for Shew_3225 in Shewanella loihica PV-4

Annotation: glutathione synthetase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 315 PF02951: GSH-S_N" amino acids 2 to 120 (119 residues), 165.2 bits, see alignment E=8.7e-53 TIGR01380: glutathione synthase" amino acids 2 to 312 (311 residues), 458 bits, see alignment E=6.6e-142 PF02955: GSH-S_ATP" amino acids 124 to 296 (173 residues), 266.8 bits, see alignment E=9.7e-84 PF08443: RimK" amino acids 142 to 309 (168 residues), 35.3 bits, see alignment E=1.3e-12

Best Hits

Swiss-Prot: 85% identical to GSHB_SHEON: Glutathione synthetase (gshB) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01920, glutathione synthase [EC: 6.3.2.3] (inferred from 100% identity to slo:Shew_3225)

MetaCyc: 74% identical to glutathione synthetase (Escherichia coli K-12 substr. MG1655)
Glutathione synthase. [EC: 6.3.2.3]

Predicted SEED Role

"Glutathione synthetase (EC 6.3.2.3)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle or Heat shock dnaK gene cluster extended (EC 6.3.2.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.3

Use Curated BLAST to search for 6.3.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QHZ3 at UniProt or InterPro

Protein Sequence (315 amino acids)

>Shew_3225 glutathione synthetase (RefSeq) (Shewanella loihica PV-4)
MIKLGIVMDPISDINIKKDSSFAMLMAAQSRGYQLYYMEMQDLAMVNGVAMGNMRRLTVK
QDPQSWYQLEEAVDTPLADLDVILMRKDPPFDTEFVYATYMLERAEEQGTLIVNKPQSLR
DANEKLFTAWFSEFTPDTIVTRDAQRIRAFYQEKGDIILKPLDGMGGSSIFRVKAQDPNL
GVIIETLTNHGQQYAMAQAFIPDITAGDKRILVVDGVPVPYSLARIPQKGETRGNLAAGG
RGVAQPLSESDWAIANAIGPELKKRGLIFVGLDVIGDKLTEINVTSPTCIREIEAAFDVD
ITGMLMDAIEARVGR