Protein Info for Shew_3206 in Shewanella loihica PV-4

Annotation: nitrate reductase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 827 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details TIGR01706: periplasmic nitrate reductase, large subunit" amino acids 3 to 827 (825 residues), 1536.5 bits, see alignment E=0 PF10518: TAT_signal" amino acids 3 to 22 (20 residues), 24 bits, see alignment (E = 5.2e-09) PF04879: Molybdop_Fe4S4" amino acids 40 to 92 (53 residues), 69.3 bits, see alignment 4.3e-23 PF00384: Molybdopterin" amino acids 96 to 568 (473 residues), 227.2 bits, see alignment E=6.3e-71 PF01568: Molydop_binding" amino acids 713 to 821 (109 residues), 97.3 bits, see alignment E=1.1e-31

Best Hits

Swiss-Prot: 76% identical to NAPA_AERS4: Periplasmic nitrate reductase (napA) from Aeromonas salmonicida (strain A449)

KEGG orthology group: K02567, periplasmic nitrate reductase NapA [EC: 1.7.99.4] (inferred from 76% identity to asa:ASA_2771)

MetaCyc: 72% identical to nitrate reductase large subunit (Aliivibrio fischeri)
Nitrate reductase (cytochrome). [EC: 1.9.6.1]

Predicted SEED Role

"Periplasmic nitrate reductase precursor (EC 1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.99.4 or 1.9.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QHX4 at UniProt or InterPro

Protein Sequence (827 amino acids)

>Shew_3206 nitrate reductase (RefSeq) (Shewanella loihica PV-4)
MSISRREFLKANAAVAAATTVGVTLPVKIVEAAEQNDNIKWDKAPCRFCGVGCSVLVGTN
NGKVVATKGDPESPVNKGLNCIKGYFLSKIMYGKDRLTTPLLRMKDGQYDKEGEFTPVSW
DKAFDTMAEKWKNTLKTKGPTAVGMFGSGQWTVWEGYAASKLHKAGFLTNNIDPNARHCM
ASAVGGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDSRLSKPNSRVH
VLSTFENRSFDLADNPMVFRPQSDLVILNFIANYIIQNGAVNKEFVSKHTKFALGTTDIG
YGLRPDHPLEKKAKNPGNGKSTAISFDEYAKFVSTYTLEYAAKMSGVEPEKLETLAKAYA
DPSVKIMSLWTMGINQHVRGVWANNMLYNIHLLTGKIATPGNSPFSLTGQPSACGTAREV
GTFAHRLPADMVVANPKHRAVTEKLWQLPEGTIPPKPGFHAVLQSRMLKDGKLNCYWTMC
TNNMQAGPNINDEIYPGFRNPDNFIVVSDPYPTVTAMAADLILPTAMWVEKEGAYGNAER
RTHMWHQQVKAPEGAKSDLWQLVEFSKRFKVSEVWPAELVAKKPEYADKTLFDVLFANGV
INKFPTSDCKGELNDESEAFGFYLQKGIFEEYAQFGRGHAHDLADFDTYHETRGLRWPVV
EGKETLRRFVEGSDPYVKAGEGYNFYGKPDGKAVIFALPYEPAAEEPNAEYDLWMSTGRV
LEHWHTGSMTARVPELYRAYPDAQIFMHPDDAKARGLKRGDEVVVASPRGEVKTRVETKG
RNKPPRGVVFMPFFDARQLVNKLLLDATDPLSKETDFKKCPVKVMKA